About: G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	This work describe a straightforward method for the isolation of clearly defined plasma membrane preparations from rat brain cortex - Percoll-purified plasma membranes, bulk of plasma membranes and low-density plasma membrane fragments. [35S]GTPγS binding experiments measured in the presence of high concentration of GDP succeeded in the resolution of the agonist-responsive component of total binding. The highest efficacy of baclofen agrees well with the role of GABA and GABA-B receptors in inhibitory signalling of the brain, the order of efficacy of different agonists of opioid receptors was DADLE DAMGO U-69593 This work describe a straightforward method for the isolation of clearly defined plasma membrane preparations from rat brain cortex - Percoll-purified plasma membranes, bulk of plasma membranes and low-density plasma membrane fragments. [35S]GTPγS binding experiments measured in the presence of high concentration of GDP succeeded in the resolution of the agonist-responsive component of total binding. The highest efficacy of baclofen agrees well with the role of GABA and GABA-B receptors in inhibitory signalling of the brain, the order of efficacy of different agonists of opioid receptors was DADLE DAMGO U-69593 (en)
Title	G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex (en)
skos:prefLabel	G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex (en)
skos:notation	RIV/67985823:_____/09:00329471!RIV10-MSM-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA309/06/0121), P(IAA500110606), P(LC06063), P(LC554), Z(AV0Z50110509), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Lisý, Václav Stöhr, Jiří Rudajev, Vladimír Svoboda, Petr Roubalová, Lenka Novotný, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	316594
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/09:00329471
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	rat cerebral cortex; plasma membrane; G-protein activity (en)
http://linked.open.../riv/klicoveSlovo	G-protein activity rat cerebral cortex plasma membrane
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0A1F55A95D1D]
http://linked.open...i/riv/nazevZdroje	Medical Science Monitor
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Neuroscience Centre Subcellular distribution of mu- and delta-opioid receptors and functional activity of trimeric G proteins after the long-term morphine stimulation. Delta-opioid receptor targeting to membrane domains: the role of palmitoylation
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Novotný, Jiří Rudajev, Vladimír Svoboda, Petr Bouřová, Lenka Lisý, Václav Stöhr, Jiří
http://linked.open...ain/vavai/riv/wos	266193900005
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka Signaling and cellular response mechanisms
issn	1234-1010
number of pages	12 (xsd:int)
is http://linked.open...avai/riv/vysledek of	G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex G-protein activity in Percoll-purified plasma membranes, bulk plasma membranes, and low-density plasma membranes isolated from rat cerebral cortex

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