About: Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Sequence-specific DNA binding is the key function through which tumor suppressor p53 exerts transactivation of the downstream target genes, often being impaired in cancer cells by mutations in the TP53 gene. Functional protein microarray technology enables a high-throughput parallel analysis of protein properties within one experiment under the same conditions. Using an array approach, we analyzed the DNA binding activity of wild type p53 protein and of 49 variants. Our results show significant differences in the binding properties between the p53 mutants. The C-terminal mutant R337C displayed the highest DNA binding activity on the array. However, the same mutant showed only a partial activation in the reporter gene assay and almost no activation of downstream target genes after transfection of expression vector into cells lacking endogenous p53 Sequence-specific DNA binding is the key function through which tumor suppressor p53 exerts transactivation of the downstream target genes, often being impaired in cancer cells by mutations in the TP53 gene. Functional protein microarray technology enables a high-throughput parallel analysis of protein properties within one experiment under the same conditions. Using an array approach, we analyzed the DNA binding activity of wild type p53 protein and of 49 variants. Our results show significant differences in the binding properties between the p53 mutants. The C-terminal mutant R337C displayed the highest DNA binding activity on the array. However, the same mutant showed only a partial activation in the reporter gene assay and almost no activation of downstream target genes after transfection of expression vector into cells lacking endogenous p53 (en)
Title	Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation (en)
skos:prefLabel	Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation (en)
skos:notation	RIV/65269705:_____/10:#0000946!RIV11-MZ0-65269705
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(NS10439), P(NS9858), Z(MSM0021622412), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	2/3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Pospíšilová, Šárka Mayer, Jiří Tichý, Boris Malčíková, Jitka Trbušek, Martin Kabáthová, Jitka
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fakultní nemocnice Brno / (nerozlišená součást)
http://linked.open...dnocenehoVysledku	246487
http://linked.open...ai/riv/idVysledku	RIV/65269705:_____/10:#0000946
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	R337C (en)
http://linked.open.../riv/klicoveSlovo	R337C
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[41BAAEABE7CD]
http://linked.open...i/riv/nazevZdroje	Biological Chemistry
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of changes in genome and its expression in chronic lymphocytic leukemia patients during the disease development Prognostic stratification of CLL patients according to genetic alterations of TP53 gene - detection of mutations using functional analysis in combination with modern approaches
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	391
http://linked.open...iv/tvurceVysledku	Mayer, Jiří Pospíšilová, Šárka Tichý, Boris Malčíková, Jitka Trbušek, Martin Kabathová, Jitka
http://linked.open...ain/vavai/riv/wos	000275194300009
http://linked.open...n/vavai/riv/zamer	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	1431-6730
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation Analysis of the DNA-binding activity of p53 mutants using functional protein microarrays and its relationship to transcriptional activation

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