About: Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole

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About: Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize beta-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin-O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows an almost complete reversion in the root elongation assay. This is the first evidence from intact plants that the vacuole is the storage organelle for CK O-glucosides and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1 as a robust molecular tool that exploits the reversibility of O-glucosylation and enables delicate manipulations of active CK content at the cellular level. Cytokinin (CK) activity is regulated by the complex interplay of their metabolism, transport, stability and cellular/tissue localization. O-glucosides of zeatin-type CKs are postulated to be storage and/or transport forms. Active CK levels are determined in part by their differential distribution of CK metabolites across different subcellular compartments. We have previously shown that overexpressing chloroplast-localized Zm-p60.1, a maize beta-glucosidase capable of releasing active cytokinins from their O- and N3-glucosides, perturbs CK homeostasis in transgenic tobacco. We obtained tobacco (Nicotiana tabacum L., cv Petit Havana SR1) plants overexpressing a recombinant Zm-p60.1 that is targeted to the vacuole. The protein is correctly processed and localized to the vacuole. When grown on medium containing exogenous zeatin, transgenic seedlings rapidly accumulate fresh weight due to ectopic growths at the base of the hypocotyl. The presence of the enzyme in these ectopic structures is shown by histochemical staining. CK quantification reveals that these transgenic seedlings are unable to accumulate zeatin-O-glucoside to levels similar to those observed in the wild type. When crossed with tobacco overexpressing the zeatin-O-glucosyltransferase gene from Phaseolus, the vacuolar variant shows an almost complete reversion in the root elongation assay. This is the first evidence from intact plants that the vacuole is the storage organelle for CK O-glucosides and that they are available to attack by Zm-p60.1. We propose the use of Zm-p60.1 as a robust molecular tool that exploits the reversibility of O-glucosylation and enables delicate manipulations of active CK content at the cellular level. (en)
Title	Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole (en)
skos:prefLabel	Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole (en)
skos:notation	RIV/62156489:43210/12:00188697!RIV13-GA0-43210___
http://linked.open...avai/predkladatel	Agronomická fakulta
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(1M06030), P(GP204/09/P289), P(IAA600380507), P(LC06034), S, Z(AV0Z50040507), Z(AV0Z50040702), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Brzobohatý, Břetislav Kiran, Nagavalli Subbanna Kuklová, Alena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	165344
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/12:00188697
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Tobacco; Storage organelle; Cytokinin metabolism; Nicotiana tabacum; Vacuole; beta-Glucosidase; Sub-cellular compartmentation; Zeatin-O-glucoside (en)
http://linked.open.../riv/klicoveSlovo	Cytokinin metabolism Tobacco Storage organelle Sub-cellular compartmentation Vacuole Zeatin-O-glucoside beta-Glucosidase Nicotiana tabacum
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[5E792C2C08D4]
http://linked.open...i/riv/nazevZdroje	Phytochemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of morphogenesis of plant cells and organs Subcellular compartmentation of reversible O-glucosylation in the homeostasis of active cytokinin levels Functional genomics and proteomics for crop improvement Mechanisms maintaining hormonal homeostasis in plant cells
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	79
http://linked.open...iv/tvurceVysledku	Malbeck, Jiří Brzobohatý, Břetislav Benková, Eva Kiran, Nagavalli Subbanna Dubová, Jaroslava Palme, Klaus Reková, Alena
http://linked.open...ain/vavai/riv/wos	307031800006
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Genome and epigenome: 1D and 3D Structure, Dynamics, Interactions with Proteins and Functions Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0031-9422
number of pages	12 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.phytochem.2012.03.012
http://localhost/t...ganizacniJednotka	43210
is http://linked.open...avai/riv/vysledek of	Retargeting a maize beta-glucosidase to the vacuole -- Evidence from intact plants that zeatin-O-glucoside is stored in the vacuole

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