About: MALDI-based intact spore mass spectrometry of downy and powdery mildews

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://onlinelibrary.wiley.com/doi/10.1002/jms.3046/pdf
Description	This study deals with a methodology for MALDI-TOF MS-based identification of downy and powdery mildews representing obligate biotrophic parasites of crop plants. Experimental approaches for the MS analyses were optimized using Bremia lactucae, cause of lettuce downy mildew, and Oidium neolycopersici, cause of tomato powdery mildew. This involved optimization of sample preparation protocols and tuning of conditions during the measurements. The best peptide/protein profiles (in terms of signal-to-noise ratio and number of peaks) were obtained by combining ferulic and sinapinic acids as a mixed MALDI matrix. A pretreatment of the spore cell wall with hydrolases was successfully introduced prior to MS measurements to obtain more pronounced signals. Finally, a novel procedure was developed for direct mass spectra acquisition from infected plant leaves. This study deals with a methodology for MALDI-TOF MS-based identification of downy and powdery mildews representing obligate biotrophic parasites of crop plants. Experimental approaches for the MS analyses were optimized using Bremia lactucae, cause of lettuce downy mildew, and Oidium neolycopersici, cause of tomato powdery mildew. This involved optimization of sample preparation protocols and tuning of conditions during the measurements. The best peptide/protein profiles (in terms of signal-to-noise ratio and number of peaks) were obtained by combining ferulic and sinapinic acids as a mixed MALDI matrix. A pretreatment of the spore cell wall with hydrolases was successfully introduced prior to MS measurements to obtain more pronounced signals. Finally, a novel procedure was developed for direct mass spectra acquisition from infected plant leaves. (en)
Title	MALDI-based intact spore mass spectrometry of downy and powdery mildews MALDI-based intact spore mass spectrometry of downy and powdery mildews (en)
skos:prefLabel	MALDI-based intact spore mass spectrometry of downy and powdery mildews MALDI-based intact spore mass spectrometry of downy and powdery mildews (en)
skos:notation	RIV/61989592:15310/12:33142960!RIV13-MSM-15310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(ED0007/01/01), P(MEB061003), Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Šebela, Marek Sedlářová, Michaela Chalupová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	147898
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/12:33142960
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein marker; plant pathogen; MALDI-TOF; intact cell/spore mass spectrometry; biotyping (en)
http://linked.open.../riv/klicoveSlovo	plant pathogen protein marker MALDI-TOF biotyping intact cell/spore mass spectrometry
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[74B1978FC03F]
http://linked.open...i/riv/nazevZdroje	Journal of Mass Spectrometry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of the Region Haná for Biotechnological and Agricultural Research Identification and biotyping of phytopathogens of cultivated plants using mass spectrometry
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	47
http://linked.open...iv/tvurceVysledku	Sedlářová, Michaela Šebela, Marek Řehulka, Pavel Allmaier, Guenter Chalupová, Jana Helmel, Michaela Marchetti-Deschmann, Martina
http://linked.open...ain/vavai/riv/wos	000307932000005
http://linked.open...n/vavai/riv/zamer	Variabilita složek a interakcí v rostlinném patosystému a vliv faktorů prostředí na jejich projev
issn	1076-5174
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jms.3046
http://localhost/t...ganizacniJednotka	15310
is http://linked.open...avai/riv/vysledek of	MALDI-based intact spore mass spectrometry of downy and powdery mildews

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