About: The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts     Goto   Sponge   NotDistinct   Permalink

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  • The present study investigated changes in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in isolated mesophyll protoplasts and cell cultures of the cucumber Cucumis sativus cv. Marketer. Although only a minor increase in the level of nitrogen oxide (NO) was observed during the first 7 days of culture following protoplast isolation, a substantial accumulation of ROS was detected. Compounds known to modulate endogenous ROS and RNS levels were employed to study their role in cucumber protoplast regeneration and growth. Supplementing the culture medium with the NO donors S-nitrosoglutathione and sodium nitroprusside and the ROS scavenger ascorbate significantly increased protoplast viability and cell density. In contrast, cell density was significantly decreased following the addition of catalase to the medium. Scavenging of ROS and RNS induced the formation of cucumber microcalli, thus suggesting a differential role of NO in the maintenance of cell viability and in the control of cell division. Our findings confirm the crucial role of controlled ROS and RNS production in both protoplast regeneration and cellular growth and differentiation.
  • The present study investigated changes in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in isolated mesophyll protoplasts and cell cultures of the cucumber Cucumis sativus cv. Marketer. Although only a minor increase in the level of nitrogen oxide (NO) was observed during the first 7 days of culture following protoplast isolation, a substantial accumulation of ROS was detected. Compounds known to modulate endogenous ROS and RNS levels were employed to study their role in cucumber protoplast regeneration and growth. Supplementing the culture medium with the NO donors S-nitrosoglutathione and sodium nitroprusside and the ROS scavenger ascorbate significantly increased protoplast viability and cell density. In contrast, cell density was significantly decreased following the addition of catalase to the medium. Scavenging of ROS and RNS induced the formation of cucumber microcalli, thus suggesting a differential role of NO in the maintenance of cell viability and in the control of cell division. Our findings confirm the crucial role of controlled ROS and RNS production in both protoplast regeneration and cellular growth and differentiation. (en)
Title
  • The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts
  • The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts (en)
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  • The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts
  • The effects of reactive nitrogen and oxygen species on the regeneration and growth of cucumber cells from isolated protoplasts (en)
skos:notation
  • RIV/61989592:15310/12:33118951!RIV13-MSM-15310___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • P(ME08048), Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika
  • 2
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  • 133484
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  • RIV/61989592:15310/12:33118951
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http://linked.open.../riv/klicovaSlova
  • Reactive oxygen species; Nitric oxide; Regeneration; Protoplast; Cucumis sativus (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [9B14DFECF77F]
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  • Plant Cell, Tissue and Organ Culture (print)
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  • 108
http://linked.open...iv/tvurceVysledku
  • Navrátilová, Božena
  • Petřivalský, Marek
  • Sedlářová, Michaela
  • Luhová, Lenka
  • Piterková, Jana
  • Ryzí, Markéta
  • Vaníčková, Pavla
http://linked.open...ain/vavai/riv/wos
  • 000299329500007
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-6857
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11240-011-0035-3
http://localhost/t...ganizacniJednotka
  • 15310
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