About: Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase

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About: Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The transformation of benzonitrile into benzohydroxamic acid was performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step was catalyzed by either a cell-free extract from Rhodococcus erythropolis A4 (nitrile hydratase) or cell-free extracts from recombinant Escherichia coli strains expressing nitrile hydratases from Raoultella terrigena 77.1 and Klebsiella oxytoca 38.1.2; the biocatalyst in the second step was a cellfree extract from R. erythropolis A4 (amidase). When using the cell-free extract from R. erythropolis A4 in the first step, the hydrolytic amidase activity was suppressed by ammonium ions, which, however, did not inhibit the acyl transfer reaction catalyzed by the same enzyme. Aromatic and aliphatic nitriles were examined as substrates of the recombinant nitrile hydratases by using a colorimetric assay of hydroxamic acids produced in a coupled acyl transfer reaction The transformation of benzonitrile into benzohydroxamic acid was performed by a cascade bienzymatic reaction involving nitrile hydration and acyl transfer of the intermediate benzamide onto hydroxylamine. The first step was catalyzed by either a cell-free extract from Rhodococcus erythropolis A4 (nitrile hydratase) or cell-free extracts from recombinant Escherichia coli strains expressing nitrile hydratases from Raoultella terrigena 77.1 and Klebsiella oxytoca 38.1.2; the biocatalyst in the second step was a cellfree extract from R. erythropolis A4 (amidase). When using the cell-free extract from R. erythropolis A4 in the first step, the hydrolytic amidase activity was suppressed by ammonium ions, which, however, did not inhibit the acyl transfer reaction catalyzed by the same enzyme. Aromatic and aliphatic nitriles were examined as substrates of the recombinant nitrile hydratases by using a colorimetric assay of hydroxamic acids produced in a coupled acyl transfer reaction (en)
Title	Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction (en)
skos:prefLabel	Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction (en)
skos:notation	RIV/61388971:_____/11:00360786!RIV12-AV0-61388971
http://linked.open...avai/predkladatel	Mikrobiologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(LC06010), P(OC09046), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Veselá, Alícia Barbara Uhnáková, Bronislava Kaplan, Ondřej Martínková, Ludmila Vejvoda, Vojtěch
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	188451
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/11:00360786
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nitrile hydratase; Rhodococcus erythropolis; Amidase (en)
http://linked.open.../riv/klicoveSlovo	Amidase Nitrile hydratase Rhodococcus erythropolis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[294607180428]
http://linked.open...i/riv/nazevZdroje	Journal of Molecular Catalysis B-Enzymatic
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Center of Biocatalysis and Biotransformation Cascade chemoenzymatic reactions of nitriles
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	71
http://linked.open...iv/tvurceVysledku	Fischer, L. Kaplan, Ondřej Lutz-Wahl, S. Martínková, Ludmila Uhnáková, Bronislava Vejvoda, Vojtěch Veselá, Alícia Barbara
http://linked.open...ain/vavai/riv/wos	000291451000008
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	1381-1177
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.molcatb.2011.03.008
is http://linked.open...avai/riv/vysledek of	Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction Biotransformation of nitriles to hydroxamic acids via a nitrile hydratase–amidase cascade reaction

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