About: Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector). Thirteen antibiotic standards and four cultivation broths were used for the method development. The comparison of ten liquid–liquid and solid phase extraction protocols for sample clean-up and pre-concentration revealed that Oasis HLB C18 sorbent gives the best recoveries. The Acquity BEH C18 chromatographic column was chosen for the samples separation with respect to its universality, selectivity, efficiency and robustness. The method is presented by two 3D fingerprints for every sample that was obtained under different, acidic and alkaline, UHPLC conditions A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector). Thirteen antibiotic standards and four cultivation broths were used for the method development. The comparison of ten liquid–liquid and solid phase extraction protocols for sample clean-up and pre-concentration revealed that Oasis HLB C18 sorbent gives the best recoveries. The Acquity BEH C18 chromatographic column was chosen for the samples separation with respect to its universality, selectivity, efficiency and robustness. The method is presented by two 3D fingerprints for every sample that was obtained under different, acidic and alkaline, UHPLC conditions (en)
Title	Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth (en)
skos:prefLabel	Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth (en)
skos:notation	RIV/61388971:_____/10:00352642!RIV11-MSM-61388971
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(2B08064), P(QH92151), S, Z(AV0Z50200510), Z(MSM0021620857), Z(MZE0002700604)
http://linked.open...iv/cisloPeriodika	51
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Plháčková, Kamila Ulanová, Dana Kameník, Zdeněk Olšovská, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	294193
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/10:00352642
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	UHPLC; high-throughput fingerprinting; secondary metabolites (en)
http://linked.open.../riv/klicoveSlovo	secondary metabolites UHPLC high-throughput fingerprinting
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6C474801AA28]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography A
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Soil microorganisms supporting cultivation conditions preventing common scab in potatoes for direct consumption. Introduction of producers generating new and modified antibiotics.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	1217
http://linked.open...iv/tvurceVysledku	Kameník, Zdeněk Kopecký, J. Olšovská, Jana Plháčková, Kamila Hadacek, F. Ulanová, Dana Marečková, M. Chobot, V.
http://linked.open...ain/vavai/riv/wos	000285429700012
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Nové molekulární systémy pro pokročilé aplikace prospěšné pro zdraví a šetrné k životnímu prostředí Udržitelné systémy pěstování zemědělských plodin pro produkci kvalitních a bezpečných potravin, krmiv a surovin
issn	0021-9673
number of pages	10 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth

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