About: Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Paper describes novel method suitable for removal of non-ionic POE-based detergents and PEG or PPG polymers. The removal is done as solid phase extraction with the peptides bound to the reversed phase resin. The detergent or polymer molecules are washed away using chlorinated solvents. Several aspects of the method were carefully tested and its sensitivity was shown. It is suitable for removal of different non-ionic detergents or polymers on peptide or protein level and it can be easily automated. Its compatibility with hydrogen/deuterium exchange was shown and demonstrated on the mapping of full membrane protein Paper describes novel method suitable for removal of non-ionic POE-based detergents and PEG or PPG polymers. The removal is done as solid phase extraction with the peptides bound to the reversed phase resin. The detergent or polymer molecules are washed away using chlorinated solvents. Several aspects of the method were carefully tested and its sensitivity was shown. It is suitable for removal of different non-ionic detergents or polymers on peptide or protein level and it can be easily automated. Its compatibility with hydrogen/deuterium exchange was shown and demonstrated on the mapping of full membrane protein (en)
Title	Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics (en)
skos:prefLabel	Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics (en)
skos:notation	RIV/61388971:_____/10:00351177!RIV11-MSM-61388971
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(KJB500200612), P(LC545), S, Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Havlíček, Vladimír Pompach, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	256264
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/10:00351177
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	mass spectrometry; proteomics; peptides (en)
http://linked.open.../riv/klicoveSlovo	mass spectrometry peptides proteomics
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[776E4E3F9D32]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Proteomic characterization of human natural killer cell membrane microdomains Functional Organisation of the Cell
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	82
http://linked.open...iv/tvurceVysledku	Forest, E. Havlíček, Vladimír Pompach, Petr Novák, P. Man, P. Mrázek, H. Brandolin, G. Pelosi, L. Rey, M.
http://linked.open...ain/vavai/riv/wos	000278616100035
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0003-2700
number of pages	10 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics Effective Removal of Nonionic Detergents in Protein Mass Spectrometry, Hydrogen/Deuterium Exchange, and Proteomics

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