| Attributes | Values |
|---|
| rdf:type
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| Description
| - Restrikčně – modifikační enzym Typu I EcoR124I je složen ze tří podjednotek v poměru HsdR2:HsdM2:HsdS1. Podjednotka HsdR je příkladem archetypu fuse mezi nukleasovou a helikasovou doménou do jednoho polypeptidu. Zkoumali jsme vztah mezi těmito fyzikálně vázanými doménami analýzou DNA translokačních vlastností komplexu EcoR124I, kde HsdR podjednotky nesly mutace v RecB-nucleasovém motivu II nebo III. Mutace v nukleasové doméně ovlivňují translokaci DNA přestože jsou od helikasové domény odděleny. Kromě redukce restrikční aktivity jsme také pozorovali sníženou rychlost translokace a ATPasové aktivity; rozdílné populace enzymů s rozdílnými charakteristikami translokační rychlosti; tendenci k zastavování během iniciace translokace; a změněnou dynamiku turnoveru HsdR (cs)
- The Type I Restriction-Modification enzyme EcoR124I comprises three subunits with the stoichiometry HsdR2:HsdM2:HsdS1. The HsdR subunits are archetypical examples of the fusion between nuclease and helicase domains into a single polypeptide, a linkage that is found in a great many other DNA processing enzymes. To explore the interrelationship between these physically-linked domains, we examined the DNA translocation properties of EcoR124I complexes in which the HsdR subunits had been mutated in the RecB-like nuclease motifs II or III. We found that nuclease mutations could have noteworthy effects on DNA translocation despite being discrete from the helicase domain. In addition to reductions in DNA cleavage activity, we also observed: decreased translocation and ATPase rates; different enzyme populations with different characteristic translocation rates; a tendency to stall during initiation; and, altered HsdR turnover dynamics
- The Type I Restriction-Modification enzyme EcoR124I comprises three subunits with the stoichiometry HsdR2:HsdM2:HsdS1. The HsdR subunits are archetypical examples of the fusion between nuclease and helicase domains into a single polypeptide, a linkage that is found in a great many other DNA processing enzymes. To explore the interrelationship between these physically-linked domains, we examined the DNA translocation properties of EcoR124I complexes in which the HsdR subunits had been mutated in the RecB-like nuclease motifs II or III. We found that nuclease mutations could have noteworthy effects on DNA translocation despite being discrete from the helicase domain. In addition to reductions in DNA cleavage activity, we also observed: decreased translocation and ATPase rates; different enzyme populations with different characteristic translocation rates; a tendency to stall during initiation; and, altered HsdR turnover dynamics (en)
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| Title
| - The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I
- The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I (en)
- Vztah helikasové a nukleasové domény během translokace DNA molekulárním motorem EcoR124I (cs)
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| skos:prefLabel
| - The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I
- The Interrelationship of Helicase and Nuclease Domains during DNA Translocation by the Molecular Motor EcoR124I (en)
- Vztah helikasové a nukleasové domény během translokace DNA molekulárním motorem EcoR124I (cs)
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| skos:notation
| - RIV/61388971:_____/08:00317626!RIV09-AV0-61388971
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA204/07/0325), Z(AV0Z50200510)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61388971:_____/08:00317626
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - single molecule; enzyme disorder; translocase (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Molecular Biology
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Weiserová, Marie
- Šišáková, Eva
- Szczelkun, M. D.
- Dekker, C.
- Seidel, R.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
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| is http://linked.open...avai/riv/vysledek
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