About: Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering

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About: Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site The gene encoding an epoxide hydrolase from Aspergillus niger M200 has been clonek and its sequence determined. Saturation mutagenesis at codon 217 resulted in the discovery of nine enzyme variants showing in several cases profound differences in activity and enantioselectivity towards various epoxides when compared to the data of the wild-type enzyme. The site 217 is located at the entrance of the tunnel that provides the substrate with access to the active site (en) Gen kódující epoxidhydrolasu z Aspergillus niger M200 byl klonován. Saturační mutageneze kodonu 217 vedla k objevení devíti variant enzymu (v některých případech) s výraznými rozdíly v aktivitě a enantioselektivitě (po porovnání s původním nezměněným enzymem). Pozice 217 je lokalizována u vchodu do substrátového tunelu, který spojuje aktivní centrum enzymu s vnějškem (cs)
Title	Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering (en) Klonování genu kódujícího epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinovým inženýrstvím (cs)
skos:prefLabel	Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering (en) Klonování genu kódujícího epoxidhydrolasu z Aspergillus niger M200, nadprodukce v E. coli a modifikace aktivity a enantioselectivity enzymu proteinovým inženýrstvím (cs)
skos:notation	RIV/61388971:_____/07:00090142!RIV08-AV0-61388971
http://linked.open.../vavai/riv/strany	8;15
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/06/0458), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Kyslík, Pavel Štěpánek, Václav Kotík, Michael Marešová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	413924
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/07:00090142
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein engineering; saturation mutagenesis; enantioselectivity (en)
http://linked.open.../riv/klicoveSlovo	enantioselectivity protein engineering saturation mutagenesis
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[992DFE955C7C]
http://linked.open...i/riv/nazevZdroje	Journal of Biotechnology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction and exploration of more effective gene libraries comprising the total microbial environmental gene pool (DNA and mRNA) of polluted sites
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	132
http://linked.open...iv/tvurceVysledku	Kyslík, Pavel Štěpánek, Václav Kotík, Michael Marešová, Helena
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology
issn	0168-1656
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering Cloning of an epoxide hydrolase-encoding gene from Aspergillus niger M200, overexpression in E. coli, and modification of aktivity and enantioselectivity of the enzyme by protein engineering

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