About: Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora

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About: Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The strain of Clostridium perfringens isolated from neonatal stools, capable of reducing bilirubin, was used in the study. Bacteria were incubated under anaerobic conditions with various native as well as synthetic bile pigments and their reduction products were analyzed by spectrophotometry, spectrofluorometry and mass spectrometry. A broad substrate activity was detected in the investigated strain of C. perfringens and a series of bilirubin reduction products was identified. Based on their physical–chemical properties, as well as mass spectra, end-catabolic bilirubin products were identified to belong to urobilinogen species. The reduction process, catalyzed enzymatically by the studied bacterial strain, does not proceed to stercobilinogen The strain of Clostridium perfringens isolated from neonatal stools, capable of reducing bilirubin, was used in the study. Bacteria were incubated under anaerobic conditions with various native as well as synthetic bile pigments and their reduction products were analyzed by spectrophotometry, spectrofluorometry and mass spectrometry. A broad substrate activity was detected in the investigated strain of C. perfringens and a series of bilirubin reduction products was identified. Based on their physical–chemical properties, as well as mass spectra, end-catabolic bilirubin products were identified to belong to urobilinogen species. The reduction process, catalyzed enzymatically by the studied bacterial strain, does not proceed to stercobilinogen (en) Kmen Clostridium perfringens izolovaný z novorozenecké fekální flóry byl použit v této studii. Pomocí spektrometrických metod byla analyzována schopnost metabolizovat rozmanité přírodní i syntetické žlučové pigmenty. Analýzou redukčních produktů vzniklých jejich konverzí jsme odhalili schopnost kmene BR1 metabolizovat dané pigmenty až na úroveň urobilinogenů, redukce na úroveň sterkobilinogenů však nepokračovala (cs)
Title	Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora (en) Identifikace redukčních produktů bilirubinu kmene Clostridium perfringens izolovaného z novorozenecké fekální flóry (cs)
skos:prefLabel	Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora (en) Identifikace redukčních produktů bilirubinu kmene Clostridium perfringens izolovaného z novorozenecké fekální flóry (cs)
skos:notation	RIV/61388971:_____/06:00050802!RIV07-GA0-61388971
http://linked.open.../vavai/riv/strany	149;157
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA310/02/1436), S, Z(AV0Z40550506), Z(AV0Z50200510)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Branny, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mikrobiologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	478701
http://linked.open...ai/riv/idVysledku	RIV/61388971:_____/06:00050802
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bilirubin; bacterial reduction; intestinal microflora (en)
http://linked.open.../riv/klicoveSlovo	bilirubin intestinal microflora bacterial reduction
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[B2208CBD6D3E]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Isolation and characterization of bilirubin reductase gene from bacteria of Clostridium genus and its potential therapeutic use
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	833
http://linked.open...iv/tvurceVysledku	Branny, Pavel Malina, J. Muchová, L. Vítek, L. Jirásková, A. Zelenka, J. Ubik, Karel Majer, F.
http://linked.open...n/vavai/riv/zamer	Regulace biologických procesů: Chemické modulátory vybraných systémů významných pro medicínu a zemědělství Microorganisms in Research and Biotechnology
issn	1570-0232
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora Identification of bilirubin reduction products formed by Clostridium perfringens isolated from human neonatal fecal flora

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