About: Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Fluorescence microscopy has become one of the most rapidly developing observation techniques in the field of molecular biology, since its high sensitivity, contrast and labeling specificity together with being non-invasive fulfill the most important requirements of live cell imaging. The biggest limitation of the technique seems to be the spatial resolution which is, based on Abbe's diffraction law, restricted to some hundreds of nanometres. Recently, various approaches have been developed that overcome the limit imposed by the diffraction barrier and these methods currently lead the development in the field of fluorescence microscopy. In this contribution, we present dynamic saturation optical microscopy (DSOM) a new technique that monitors the temporal decay of the excited singlet state due to a dark state formation. By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained. Fluorescence microscopy has become one of the most rapidly developing observation techniques in the field of molecular biology, since its high sensitivity, contrast and labeling specificity together with being non-invasive fulfill the most important requirements of live cell imaging. The biggest limitation of the technique seems to be the spatial resolution which is, based on Abbe's diffraction law, restricted to some hundreds of nanometres. Recently, various approaches have been developed that overcome the limit imposed by the diffraction barrier and these methods currently lead the development in the field of fluorescence microscopy. In this contribution, we present dynamic saturation optical microscopy (DSOM) a new technique that monitors the temporal decay of the excited singlet state due to a dark state formation. By mapping the intensity dependent decay kinetics, enhanced resolution images can be obtained. (en)
Title	Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement (en)
skos:prefLabel	Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement (en)
skos:notation	RIV/61388955:_____/10:00349037!RIV11-MSM-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GEMEM/09/E006), P(KJB400400904), P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	39
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hof, Martin Macháň, Radek Humpolíčková, Jana Benda, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	255505
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/10:00349037
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	fluorescence microscopy; dunamic saturation optical microscopy; fluorescence (en)
http://linked.open.../riv/klicoveSlovo	dunamic saturation optical microscopy fluorescence fluorescence microscopy
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[0979EACF83AF]
http://linked.open...i/riv/nazevZdroje	Physical Chemistry Chemical Physics
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Development of modular spectral confocal fluorescence microscope with enhanced spatial resolution, using combined EM-CCD and SPAD detection, and offering special FCS-based point measurement modes. Flurescence microscopy in biological and medical research Molecular level physiology and pathology of oxidized phospholipids
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	12
http://linked.open...iv/tvurceVysledku	Hof, Martin Humpolíčková, Jana Macháň, Radek Benda, Aleš Enderlein, J.
http://linked.open...ain/vavai/riv/wos	000282643900013
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	1463-9076
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement Dynamic saturation optical microscopy: employing dark-state formation kinetics for resolution enhancement

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