About: Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching by light absorbing surfaces. Applied to SPBs this effect leads to strong differences in fluorescence lifetimes when the dye moves from the outer lipid leaflet to the leaflet in contact with the support. Herein, we present the basic principles of this novel approach, and comment on its advantages over the commonly used methods for investigating flip-flop dynamics across lipid bilayers. We test the assay on the fluorescent lipid analog Atto633-DOPE and the 3-hydroxyflavone F2N12S probe in SPBs composed of DOPC/ DOPS lipids. Moreover, we compare and discuss the flip-flop rates of the probes with respect to their lateral diffusion coefficients. In the present work we introduce a straightforward fluorescent assay that can be applied in studies of the transbilayer movement (flip-flop) of fluorescent lipid analogues across supported phospholipid bilayers (SPBs). The assay is based on the distance dependent fluorescence quenching by light absorbing surfaces. Applied to SPBs this effect leads to strong differences in fluorescence lifetimes when the dye moves from the outer lipid leaflet to the leaflet in contact with the support. Herein, we present the basic principles of this novel approach, and comment on its advantages over the commonly used methods for investigating flip-flop dynamics across lipid bilayers. We test the assay on the fluorescent lipid analog Atto633-DOPE and the 3-hydroxyflavone F2N12S probe in SPBs composed of DOPC/ DOPS lipids. Moreover, we compare and discuss the flip-flop rates of the probes with respect to their lateral diffusion coefficients. (en)
Title	Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers (en)
skos:prefLabel	Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers (en)
skos:notation	RIV/61388955:_____/10:00342761!RIV11-MSM-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GEMEM/09/E006), P(LC06063), Z(AV0Z40400503)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Hof, Martin Jurkiewicz, Piotr Sýkora, Jan Benda, Aleš Kulakowska-Zań, Anna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	259448
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/10:00342761
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	flip-flop dynamics; lateral diffusion; fluorescence lifetime tuning (en)
http://linked.open.../riv/klicoveSlovo	flip-flop dynamics fluorescence lifetime tuning lateral diffusion
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E2A1A4B2C990]
http://linked.open...i/riv/nazevZdroje	Journal of Fluorescence
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Molecular level physiology and pathology of oxidized phospholipids
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	20
http://linked.open...iv/tvurceVysledku	Hof, Martin Jurkiewicz, Piotr Sýkora, Jan Benda, Aleš Kulakowska-Zań, Anna Mely, Y.
http://linked.open...ain/vavai/riv/wos	000275421600016
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace
issn	1053-0509
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers Fluorescence Lifetime Tuning-A Novel Approach to Study Flip-Flop Kinetics in Supported Phospholipid Bilayers

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