About: The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

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About: The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of non-ionic detergents on baclofen (GABA(B)-R agonist)-stimulated G-protein activity was measured as a [S-35] GTP gamma S,IS binding assay in the plasma membranes (PM) isolated from the brain tissue. The effect was clearly biphasic - a decrease in the activity was followed by an activation maximum and finally, at high concentrations, drastic inhibition of the G-protein activity was noticed. Contrarily, specific radioligand binding to GABA(B)-receptor was inhibited in the whole range of detergent concentrations step by step, i.e. it was strictly monophasic. The magnitude of both detergent effects was decreased in the same order of potency: Brij58>Triton X-100>Digitonin. The identical order was found when comparing detergents ability to alter fluorescence anisotropy of the membrane probe 1,6-diphenyl-1,3,5-hexatriene (r (DPH)) incorporated into the hydrophobic PM interior. The effect of non-ionic detergents on baclofen (GABA(B)-R agonist)-stimulated G-protein activity was measured as a [S-35] GTP gamma S,IS binding assay in the plasma membranes (PM) isolated from the brain tissue. The effect was clearly biphasic - a decrease in the activity was followed by an activation maximum and finally, at high concentrations, drastic inhibition of the G-protein activity was noticed. Contrarily, specific radioligand binding to GABA(B)-receptor was inhibited in the whole range of detergent concentrations step by step, i.e. it was strictly monophasic. The magnitude of both detergent effects was decreased in the same order of potency: Brij58>Triton X-100>Digitonin. The identical order was found when comparing detergents ability to alter fluorescence anisotropy of the membrane probe 1,6-diphenyl-1,3,5-hexatriene (r (DPH)) incorporated into the hydrophobic PM interior. (en)
Title	The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence (en)
skos:prefLabel	The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence (en)
skos:notation	RIV/61388955:_____/09:00333814!RIV10-MSM-61388955
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA309/06/0121), P(IAA500110606), P(LC06063), P(LC554), Z(AV0Z40400503), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hof, Martin Sýkora, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fyzikální chemie J. Heyrovského AV ČR, v.v.i.
http://linked.open...dnocenehoVysledku	312055
http://linked.open...ai/riv/idVysledku	RIV/61388955:_____/09:00333814
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	detergent; fluorescence; stready-state; time-resolved (en)
http://linked.open.../riv/klicoveSlovo	fluorescence detergent time-resolved stready-state
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[604AFDA0A679]
http://linked.open...i/riv/nazevZdroje	Biochimica Et Biophysica Acta-Biomembranes
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Flurescence microscopy in biological and medical research Neuroscience Centre Subcellular distribution of mu- and delta-opioid receptors and functional activity of trimeric G proteins after the long-term morphine stimulation. Delta-opioid receptor targeting to membrane domains: the role of palmitoylation
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	1788
http://linked.open...iv/tvurceVysledku	Hof, Martin Svoboda, Petr Sýkora, Jan Bouřová, Lenka
http://linked.open...ain/vavai/riv/wos	000263794700004
http://linked.open...n/vavai/riv/zamer	Struktura, reaktivita a dynamika molekulárních a biomolekulárních systémů: teorie, experiment, aplikace Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	0005-2736
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

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