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| rdf:type
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| Description
| - Mason-Pfizer monkey virus encodes an aspartic protease (M-PMV PR), which is essential for the correct assembly and maturation of the viral particles. Primary sequence of the protease contains two cysteine residues (Cys7, Cys106) that has been replaced by alanines to stabilize the monomeric form of the 12 kDa protease for structural study by NMR spectroscopy [1]. However, it was demonstrated that cysteines were not indifferent amino acid residues and might have a modulatory affect on the activity of the protease [2]. Here we present results of structural and biochemical study on the inactive mutants (D26N) of the 12 kDa form of M-PMV PR with either one (C7A, C106A) or both cysteine residues preserved. We demonstrate that replacement of both cysteines affects not only the stability of the protease but also the proteolytic activity of the enzyme. MALDI-TOF measurements of a digest of PRD26N and a light scattering analysis revealed the formation of an intramolecular disulphide bridge, which might help to
- Mason-Pfizer monkey virus encodes an aspartic protease (M-PMV PR), which is essential for the correct assembly and maturation of the viral particles. Primary sequence of the protease contains two cysteine residues (Cys7, Cys106) that has been replaced by alanines to stabilize the monomeric form of the 12 kDa protease for structural study by NMR spectroscopy [1]. However, it was demonstrated that cysteines were not indifferent amino acid residues and might have a modulatory affect on the activity of the protease [2]. Here we present results of structural and biochemical study on the inactive mutants (D26N) of the 12 kDa form of M-PMV PR with either one (C7A, C106A) or both cysteine residues preserved. We demonstrate that replacement of both cysteines affects not only the stability of the protease but also the proteolytic activity of the enzyme. MALDI-TOF measurements of a digest of PRD26N and a light scattering analysis revealed the formation of an intramolecular disulphide bridge, which might help to (en)
- Mason-Pfizer monkey virus encodes an aspartic protease (M-PMV PR), which is essential for the correct assembly and maturation of the viral particles. Primary sequence of the protease contains two cysteine residues (Cys7, Cys106) that has been replaced by alanines to stabilize the monomeric form of the 12 kDa protease for structural study by NMR spectroscopy [1]. However, it was demonstrated that cysteines were not indifferent amino acid residues and might have a modulatory affect on the activity of the protease [2]. Here we present results of structural and biochemical study on the inactive mutants (D26N) of the 12 kDa form of M-PMV PR with either one (C7A, C106A) or both cysteine residues preserved. We demonstrate that replacement of both cysteines affects not only the stability of the protease but also the proteolytic activity of the enzyme. MALDI-TOF measurements of a digest of PRD26N and a light scattering analysis revealed the formation of an intramolecular disulphide bridge, which might help to (cs)
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| Title
| - Affect of cysteines on the activity and structure of 12 kda form of m-pmv protease
- Affect of cysteines on the activity and structure of 12 kda form of m-pmv protease (en)
- Vlliv cysteinů na aktivitu a strukturu 12kDa formy proteasy M-PMV (cs)
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| skos:prefLabel
| - Affect of cysteines on the activity and structure of 12 kda form of m-pmv protease
- Affect of cysteines on the activity and structure of 12 kda form of m-pmv protease (en)
- Vlliv cysteinů na aktivitu a strukturu 12kDa formy proteasy M-PMV (cs)
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| skos:notation
| - RIV/60461373:22330/05:00015783!RIV06-AV0-22330___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60461373:22330/05:00015783
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Mason-Pfizer monkey virus; aspartic protease; NMR spectroscopy (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Pichová, Iva
- Hrabal, Richard
- Bauerová, Helena
- Svatoš, Antonín
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| http://localhost/t...ganizacniJednotka
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