About: Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers

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Description	Bacterial canker of tomato, caused by bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is a well-known disease resulting in serious losses to both greenhouse and field tomato crops in areas where tomatoes are planted. Cmm was detected and isolated from various parts of strongly infected plants of 10 indeterminate tomato cultivars. The most sensitive technique was optimized PCR with Dreier´s and laboratory-generated PCR primers from the Cmm tomatinase gene. All samples taken from base, stem and petiole of tomato plants were positive in PCR with both primer pairs, and also using IIF and PTA-ELISA in contrast to the samples taken from leaves. Using IIF and PTA-ELISA with PAbs of Neogen Europe Ltd. (UK), Cmm was reliably detected to dilution 1:1000 and 1:100 respectively. Seeds of 4 indeterminate and 4 determinate cultivars were consecutively artificially infected by the mixture of the two reference Cmm strains (BCCM/LMG 7333 and 5727) soaking 48 hours in inoculum (108 CFU/ml). Bacterial canker of tomato, caused by bacterium Clavibacter michiganensis subsp. michiganensis (Cmm) is a well-known disease resulting in serious losses to both greenhouse and field tomato crops in areas where tomatoes are planted. Cmm was detected and isolated from various parts of strongly infected plants of 10 indeterminate tomato cultivars. The most sensitive technique was optimized PCR with Dreier´s and laboratory-generated PCR primers from the Cmm tomatinase gene. All samples taken from base, stem and petiole of tomato plants were positive in PCR with both primer pairs, and also using IIF and PTA-ELISA in contrast to the samples taken from leaves. Using IIF and PTA-ELISA with PAbs of Neogen Europe Ltd. (UK), Cmm was reliably detected to dilution 1:1000 and 1:100 respectively. Seeds of 4 indeterminate and 4 determinate cultivars were consecutively artificially infected by the mixture of the two reference Cmm strains (BCCM/LMG 7333 and 5727) soaking 48 hours in inoculum (108 CFU/ml). (en)
Title	Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers (en)
skos:prefLabel	Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers (en)
skos:notation	RIV/60077344:_____/11:00372946!RIV12-AV0-60077344
http://linked.open...avai/predkladatel	Biologické centrum AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH71229), Z(AV0Z50510513)
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Mráz, Ivan Beran, Pavel
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	193697
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/11:00372946
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	bacterial canker of tomato; ELISA; PCR; IF; detection (en)
http://linked.open.../riv/klicoveSlovo	ELISA PCR detection IF bacterial canker of tomato
http://linked.open...ontrolniKodProRIV	[9640CCC5D8ED]
http://linked.open...v/mistoKonaniAkce	Ischia, Naples
http://linked.open...i/riv/mistoVydani	Leuven
http://linked.open...i/riv/nazevZdroje	III International Symposium on Tomato Diseases
http://linked.open...in/vavai/riv/obor	GF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Diagnostics and methods of protection against quarantine and other economically important pathogens of fruit and leafy vegetables
http://linked.open...UplatneniVysledku	2011
http://linked.open...iv/tvurceVysledku	Beran, Pavel Kokošková, B. Mráz, Ivan
http://linked.open...vavai/riv/typAkce	WRD - Světová
http://linked.open.../riv/zahajeniAkce	2010-07-25 (xsd:date)
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny
number of pages	4 (xsd:int)
http://purl.org/ne...btex#hasPublisher	ISHS Acta Horticulturae
https://schema.org/isbn	9789066056145
is http://linked.open...avai/riv/vysledek of	Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers Detection of Clavibacter michiganensis subsp. michiganensis from tomato plants and seeds using ELISA, IF and PCR with commercial and own primers

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