About: A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an economically advantageous alternative to recently established protocol for isotopic labeling using expensive synthetic media. The method is based on cultivation of the L. tarentolae expression strain in a cheap complex medium supplemented with labeled amino acid(s). In this protocol, a labeled amino acid is deliberately diluted in the medium of undefined composition, which leads to alow-level isotope enrichment upon protein over-expression. The economic advantage of the protocol is achieved by avoiding large volumes of expensive synthetic medium. Decreased sensitivity of a NMR experiment due to low-level isotope enrichment is compensated by a five- to seven-fold increase of the yield of the recombinant protein in complex mediumas compared to that in the synthetic medium. We report a cost efficient approach for amino-acid-type selective isotope labeling of proteins expressed in Leishmania tarentolae. The method provides an economically advantageous alternative to recently established protocol for isotopic labeling using expensive synthetic media. The method is based on cultivation of the L. tarentolae expression strain in a cheap complex medium supplemented with labeled amino acid(s). In this protocol, a labeled amino acid is deliberately diluted in the medium of undefined composition, which leads to alow-level isotope enrichment upon protein over-expression. The economic advantage of the protocol is achieved by avoiding large volumes of expensive synthetic medium. Decreased sensitivity of a NMR experiment due to low-level isotope enrichment is compensated by a five- to seven-fold increase of the yield of the recombinant protein in complex mediumas compared to that in the synthetic medium. (en)
Title	A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae (en)
skos:prefLabel	A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae (en)
skos:notation	RIV/60077344:_____/09:00334906!RIV10-MSM-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1QS600220554), P(2B06129), P(GP204/08/P585), P(KAN200100801), Z(AV0Z60220518), Z(MSM6007665801)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Trantírek, Lukáš Lukeš, Julius Foldynová-Trantírková, Silvie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	301222
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/09:00334906
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	NMR; isotope labeling; protein expression; Leishmania; low-level enrichment (en)
http://linked.open.../riv/klicoveSlovo	Leishmania low-level enrichment NMR protein expression isotope labeling
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[A3F0483E5D9D]
http://linked.open...i/riv/nazevZdroje	Journal of Biomolecular Structure & Dynamics
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Construction of the New Eukaryotic Expression System Model organisms as a key to the understanding of functions of disease-related human genes Bioactive Biocompatible Surfaces and Novel Nanostructured Composites for Applications to Medicine and Drug Delivery Functional analysis of methyltransferase involved in SL RNA modification in Trypanosoma brucei
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Lukeš, Julius Foldynová-Trantírková, Silvie Trantírek, Lukáš Breitling, R. Alexandov, K. Cirstea, I. Dötsch, V. Löhr, F. Matulová, J.
http://linked.open...ain/vavai/riv/wos	000266300700008
http://linked.open...n/vavai/riv/zamer	Parazitismus a parazito-hostitelské vztahy na organismální, buněčné a molekulové úrovni EKOLOGICKÉ, EVOLUČNÍ A EXPERIMENTÁLNĚ BIOLOGICKÉ PŘÍSTUPY KE STUDIU VZNIKU A VÝZNAMU BIODIVERZITY
issn	0739-1102
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae A Cost-effective Amino-acid-type Selective Isotope Labeling of Proteins Expressed in Leishmania tarentolae

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 75 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software