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| rdf:type
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| Description
| - Komplex Leishmania donovani se skládá z L. donovani, L. infantum a L. archibaldi, ačkoli posledně jmenovaný zástupce není považován za validní druh. Rozlišení leishmánií na základě morfologie není možné; používají se biochemické, imunologické a molekulárně biologické přístupy. Multilokální enzymová elektroforéza (MLEE) je v současnosti tzv. zlatým standardem. Sekvenovali jsme geny kodující 5 metabolických enzymů používaných pro MLEE, a to pro rozlišení DNA diverzity příslušných isozymů a rovněž pro posouzení jejich vhodnosti pro multilokální sekvenování na bázi PCR. U 17 kmenů L. infantum, 7 kmenů L. donovani a 3 kmenů L. archibaldi byly sekvenovány geny pro isocitrate dehydrogenázu, jablečný enzym, manosofosfát isolerázu, glukoso-6-fosfát dehydrogenázu a fumarát hydratázu. Fylogenetická analýza založená na 263 kb protein-kodujících sekvencí ukázala silnou korelaci mezi genotypem a geografickým původem. Evropa a Afrika představují na základě našich výsledků nezávislá evoluční centra. (cs)
- The complex Leishmania donovani is comprised of L. donovani (LD), L. infantum (LI) and L. archibaldi (LA). Morphological distinction of species is impractical, so biochemical, immunological and DNA-based criteria were introduced. Multilocus enzyme electrophoresis (MLEE) is the present gold standard. We have sequenced the genes encoding 5 metabolic enzymes used for MLEE, both to resolve the DNA diversity underlying isoenzyme mobility differences and to explore the potential of these targets for higher resolution PCR-based multilocus sequence typing. The genes sequenced were isocitrate dehydrogenase, malic enzyme, mannose phosphate isomerase, glucose-6-phosphate dehydrogenase, and fumarate hydratase, for 17 LI strains, 7 LD strains , and 3 LA strains. Phylogenetic analysis of a concatenated alignment based on a total of 263 kb protein-coding sequences showed strong correlation of genotype with geographical origin. Europe and Africa appear to represent independent evolutionary centres.
- The complex Leishmania donovani is comprised of L. donovani (LD), L. infantum (LI) and L. archibaldi (LA). Morphological distinction of species is impractical, so biochemical, immunological and DNA-based criteria were introduced. Multilocus enzyme electrophoresis (MLEE) is the present gold standard. We have sequenced the genes encoding 5 metabolic enzymes used for MLEE, both to resolve the DNA diversity underlying isoenzyme mobility differences and to explore the potential of these targets for higher resolution PCR-based multilocus sequence typing. The genes sequenced were isocitrate dehydrogenase, malic enzyme, mannose phosphate isomerase, glucose-6-phosphate dehydrogenase, and fumarate hydratase, for 17 LI strains, 7 LD strains , and 3 LA strains. Phylogenetic analysis of a concatenated alignment based on a total of 263 kb protein-coding sequences showed strong correlation of genotype with geographical origin. Europe and Africa appear to represent independent evolutionary centres. (en)
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| Title
| - The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing
- The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing (en)
- Komplex Leishmania donovani: genotypy pěti metabolických enzymů (ICD, ME, MPI, G6PDH and FH), nových cílů multilokálního sekvenčního typování (cs)
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| skos:prefLabel
| - The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing
- The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing (en)
- Komplex Leishmania donovani: genotypy pěti metabolických enzymů (ICD, ME, MPI, G6PDH and FH), nových cílů multilokálního sekvenčního typování (cs)
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| skos:notation
| - RIV/60077344:_____/07:00087493!RIV08-AV0-60077344
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(2B06129), R, S, Z(AV0Z60220518), Z(MSM6007665801)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00087493
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Leishmania donovani complex; zymodeme; multilocus sequence typing; Leishmania; phylogenetic network (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - AU - Australské společenství
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - International Journal for Parasitology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Horák, Aleš
- Jirků, Milan
- Lukeš, Julius
- Zemanová, Eva
- Mauricio, I. L.
- Miles, M. A.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
of | - The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing
- The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing
- The Leishmania donovani complex: Genotypes of five metabolic enzymes (ICD, ME, MPI, G6PDH and FH), new targets for multilocus sequence typing
|
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