About: Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin     Goto   Sponge   NotDistinct   Permalink

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  • U obaleče jablečného, Cydia pomonella, jsme pomocí laserové mikrodisekce sex chromatinu a následné DOP-PCR jsme získali vzorek sekvencí DNA pohlavního chromosomu W. Tento vzorek jsme využili pro přípravu W-specifické malovací sondy pro fluorescenční in situ hybridizaci (FISH) a pro získání primárních sekvenčních dat z chromosomu W. Prokázali jsme, že heterochromatinový chromosom W je převážně tvořen repetitivními sekvencemi dvou typů (akumulované na W a všudypřítomné), ale obsahuje i sekvence unikátní. Naše výsledky ukazují, že složení chromosomu W u C. pomonella není principiálně odlišné od W u Bombyx mori nebo od chromosomu Y u druhů se systémem determinace pohlaví typu XY. (cs)
  • In the codling moth, Cydia pomonella, we collected chromatin samples by laser microdissection from W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. We found that the W chromosome has attracted during evolution repetitive sequences (mainly multi-copy sequences enriched in the W chromosome and ubiquitous multi-copy sequences) but also contains unique sequences. The results show that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism.
  • In the codling moth, Cydia pomonella, we collected chromatin samples by laser microdissection from W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. We found that the W chromosome has attracted during evolution repetitive sequences (mainly multi-copy sequences enriched in the W chromosome and ubiquitous multi-copy sequences) but also contains unique sequences. The results show that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism. (en)
Title
  • Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin
  • Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin (en)
  • Průzkum chromosomu W obaleče jablečného, Cydia pomonella, se sekvencemi získanými mikrodisekcí sex chromatinu (cs)
skos:prefLabel
  • Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin
  • Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin (en)
  • Průzkum chromosomu W obaleče jablečného, Cydia pomonella, se sekvencemi získanými mikrodisekcí sex chromatinu (cs)
skos:notation
  • RIV/60077344:_____/07:00044603!RIV07-AV0-60077344
http://linked.open.../vavai/riv/strany
  • 135;145
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/06/1860), P(GD521/03/H160), P(IAA6007307), V, Z(AV0Z50070508), Z(MZE0002716201)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 444568
http://linked.open...ai/riv/idVysledku
  • RIV/60077344:_____/07:00044603
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • W chromosome (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [CC40EBCEB943]
http://linked.open...i/riv/nazevZdroje
  • Chromosoma
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 116
http://linked.open...iv/tvurceVysledku
  • Marec, František
  • Vítková, Magda
  • Traut, W.
  • Kubíčková, S.
  • Nguyen, Petr
  • Fuková, Iva
http://linked.open...n/vavai/riv/zamer
issn
  • 0009-5915
number of pages
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