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rdf:type
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Description
| - U obaleče jablečného, Cydia pomonella, jsme pomocí laserové mikrodisekce sex chromatinu a následné DOP-PCR jsme získali vzorek sekvencí DNA pohlavního chromosomu W. Tento vzorek jsme využili pro přípravu W-specifické malovací sondy pro fluorescenční in situ hybridizaci (FISH) a pro získání primárních sekvenčních dat z chromosomu W. Prokázali jsme, že heterochromatinový chromosom W je převážně tvořen repetitivními sekvencemi dvou typů (akumulované na W a všudypřítomné), ale obsahuje i sekvence unikátní. Naše výsledky ukazují, že složení chromosomu W u C. pomonella není principiálně odlišné od W u Bombyx mori nebo od chromosomu Y u druhů se systémem determinace pohlaví typu XY. (cs)
- In the codling moth, Cydia pomonella, we collected chromatin samples by laser microdissection from W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. We found that the W chromosome has attracted during evolution repetitive sequences (mainly multi-copy sequences enriched in the W chromosome and ubiquitous multi-copy sequences) but also contains unique sequences. The results show that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism.
- In the codling moth, Cydia pomonella, we collected chromatin samples by laser microdissection from W-chromatin bodies. DNA from the samples was amplified by degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) and used to prepare painting probes and start an analysis of the W-chromosome sequence composition. We found that the W chromosome has attracted during evolution repetitive sequences (mainly multi-copy sequences enriched in the W chromosome and ubiquitous multi-copy sequences) but also contains unique sequences. The results show that the W-chromosome composition of C. pomonella is not principally different from that of Bombyx mori or from that of Y chromosomes of several species with an XY sex-determining mechanism. (en)
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Title
| - Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin
- Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin (en)
- Průzkum chromosomu W obaleče jablečného, Cydia pomonella, se sekvencemi získanými mikrodisekcí sex chromatinu (cs)
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skos:prefLabel
| - Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin
- Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin (en)
- Průzkum chromosomu W obaleče jablečného, Cydia pomonella, se sekvencemi získanými mikrodisekcí sex chromatinu (cs)
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skos:notation
| - RIV/60077344:_____/07:00044603!RIV07-AV0-60077344
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA206/06/1860), P(GD521/03/H160), P(IAA6007307), V, Z(AV0Z50070508), Z(MZE0002716201)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/07:00044603
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Marec, František
- Vítková, Magda
- Traut, W.
- Kubíčková, S.
- Nguyen, Petr
- Fuková, Iva
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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is http://linked.open...avai/riv/vysledek
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