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| Description
| - Lepidoptera possess a WZ/ZZ (felame/male) sex chromosome system or its numerical variations. However, little is known about the sex-determining mechanism in this large insect order. Our recent results, obtained by comparative genomic hybridization (CGH) in Bombyx mori, Ephestia kuehniella, and Galleria mellonella suggest an accumulation of common repetitive sequences in the W chromosome. In addition, we found a male killing activity in a W-chromosome segment of Ephestia mutants. We propose here a detailed analysis of the W chromosome in selected species by methods of molecular cytogenetics. We intend to study molecular differentiation of the W by CGH, molecular composition of the W by fluorescence in situ hybridization (FISH) mapping of probes derived either from Bombyx retrotransposones or from W-chromatin, and pattern of histone acetylation in W and Z.It is expected that results obtained will contribute to understanding the role of W chromosome in sex determination of Lepidoptera. (en)
- Lepidoptera mají WZ/ZZ (samice/samec) systém pohlavních chromosomĎ nebo jeho numerické variace. Avçak velmi málo je známo o mechanismu determinace pohlaví u tohoto velkého ýádu hmyzu. Naçe nedávné věsledky, získané komparativní genomovou hybridizací (CGH) u Bombyx mori, Ephestia kuehniella a Galleria mellonella, indikují akumulací bŘÔněch repetitivních sekvencí v chromosomu W. TéÔ jsme v segmentu W chromosomu u mutantu Ephestia prokázali pýítomnost faktoru letálního pro samčí pohlaví. Navrhujeme detailní analězu chromosomu W u vybraněch druhů metodami molekulární cytogenetiky. Hodláme studovat molekulární diferenciaci W pomocí CGH, molekulární složení W mapování sond odvozeněch z retrotransposonů B. mori či z W-chromatinu fluorescenční in situ hybridizací (FISH), a také acetylaci histonů u W a Z. Očekáváme, že získané výsledky přispějí k poznání role chromosomu W v determinaci pohlaví motýlů.
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| Title
| - Role of the sex chromosome W in sex determination of Lepidoptera (en)
- Role pohlavního chromosomu W v determinaci pohlaví motýlů
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - Lepidoptera; sex chromosomes; sex determination; heterochromatin; W chromatin; molecular cytogenetics; comparative genomic hybridization; CGH; fluorescence in situ hybridization; FISH; repetitive DNA sequences; retrotransposones; histone acetylation (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...okUkonceniPodpory
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| http://linked.open...okZahajeniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...jektu+dodavatelem
| - Projekt přinesl nové poznatky o struktuře, molekulární diferenciaci a molekulárním složení pohlavního chromosomu W motýlů. Získané výsledky znamenají značný pokrok v chápání evoluce chromosomu W a jeho role v determinaci pohlaví. (cs)
- The project brought original data on structure, molecular differentiation and composition of the W chromosome in Lepidoptera. Results obtained represent a significant progress in understanding the W chromosome evolution and its role in sex determination. (en)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - Lepidoptera
- FISH
- comparative genomic hybridization
- fluorescence in situ hybridization
- heterochromatin
- molecular cytogenetics
- sex chromosomes
- sex determination
- CGH
- W chromatin
- repetitive DNA sequences
- retrotransposones
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| is http://linked.open...vavai/riv/projekt
of | - Resolution of sex chromosome constitution by genomic in situ hybridization and fluorescence in situ hybridization with (TTAGG)(n) telomeric probe in some species of Lepidoptera
- Development of genetic sexing strains in Lepidoptera: from traditional to transgenic approaches
- Karyotype, sex chromatin and sex chromosome differentiation in the carob moth, Ectomyelois ceratoniae (Lepidoptera: Pyralidae)
- Codling moth cytogenetics: karyotype, chromosomal location of rDNA, and molecular differentiation of sex chromosomes
- Analysis of radiation-induced W Chromosome aberrations in the codling moth, Cydia pomonella (L.), by fluorescence in situ hybridization techniques
- Probing the W chromosome of the codling moth, Cydia pomonella, with sequences from microdissected sex chromatin
- Divergent location of ribosomal genes in chromosomes of fish thorny-headed worms, Pomphorhynchus laevis and Pomphorhynchus tereticollis (Acanthocephala)
- FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidoptera by means of B. mori W-BAC probes
- Developing transgenic sexing strains for the release of non-transgenic sterile male codling moths Cydia pomonella
- Moth sex chromatin probed by comparative genomic hybridization (CGH).
- Codling moth cytogenetics: karyotype, sex-chromosome differentiation and development of W-chromosome painting probes
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| is http://linked.open...vavai/cep/projekt
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