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| Description
| - Metabolism of the solvents N,N-dimethylformamide (DMF) and N-methylformamide (MF) results in the formation of N-methylcarbamoyl adducts at the N-terminal valine and lysine in blood protein globin. Globin from exposed subjects to DMF (n = 35) and unexposed controls (n = 5) was analyzed by 2 methods. Edman degradation was used as to measure the valine adduct by converting it to 3-methyl-5-isopropylhydantoin (MVH). The MVH levels in globin of the exposed subjects were in the range of 1-441 nmol/g, in controls < 1 nmol/g. The principal method involved enzymatic hydrolysis with pronase to release free N-epsilon-(N-methylcarbamoyl)lysine (MLU) and N-methyl-carbamoylvaline (MVU), which were directly determined by HPLC/MS/MS. MLU was found in most globins from the exposed subjects but not in the controls. A close correlation between the MLU and MVH levels (nmol/g) was observed: MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32).
- Metabolism of the solvents N,N-dimethylformamide (DMF) and N-methylformamide (MF) results in the formation of N-methylcarbamoyl adducts at the N-terminal valine and lysine in blood protein globin. Globin from exposed subjects to DMF (n = 35) and unexposed controls (n = 5) was analyzed by 2 methods. Edman degradation was used as to measure the valine adduct by converting it to 3-methyl-5-isopropylhydantoin (MVH). The MVH levels in globin of the exposed subjects were in the range of 1-441 nmol/g, in controls < 1 nmol/g. The principal method involved enzymatic hydrolysis with pronase to release free N-epsilon-(N-methylcarbamoyl)lysine (MLU) and N-methyl-carbamoylvaline (MVU), which were directly determined by HPLC/MS/MS. MLU was found in most globins from the exposed subjects but not in the controls. A close correlation between the MLU and MVH levels (nmol/g) was observed: MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32). (en)
- Metabolismus rozpouštědel N,N-dimethylformamidu (DMF) a N-methylformamidu (MF) vede ke vzniku N-methylkarbamoylových aduktů s krevním globinem na N-terminálovém valinu a lysinu. Globin osob vystavených DMF (n = 35 ) a kontroly (n = 5) byly analyzovány dvěma metodami. Edmanova degradace byla použita k měření valinového aduktu 3-methyl-5-isopropylhydantoinu (MVH). MVH hladiny u exponovaných osob byly v rozmezí 1-441 nmol/g, v kontrolách < 1 nmol/g. Hlavní použitá metoda zahrnovala enzymatickou pronázovou hydrolýzu a následnou HPLC/M/MS analýzu N-epsilon-(N-methylkarbamoyl)lysinu (MLU) and N-methyl-karbamoylvaline (MVU). MLU byl nalezen ve většině globinů u exponovaných subjektů, ale ne v kontrolách. Byla pozorována blízká korelace mezi hladinami MLU a MVH (nmol/g) : MLU = 7 + 0.48 MVH (R-2 = 0.84, n = 32). Poznatky naznačují dlouhodobou persitenci MLU v lidském těle, a tak jeho potenciál posloužit jako biomarker chronické expozice DMF. (cs)
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| Title
| - N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans
- Adukt N-Methylkarbamoyl-lysinu v hemoglobinu: nový metabolický produkt a potenciální biomarker expozice N,N-dimethylformamidu u člověka (cs)
- N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans (en)
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| skos:prefLabel
| - N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans
- Adukt N-Methylkarbamoyl-lysinu v hemoglobinu: nový metabolický produkt a potenciální biomarker expozice N,N-dimethylformamidu u člověka (cs)
- N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans (en)
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| skos:notation
| - RIV/60077344:_____/06:00047087!RIV07-AV0-60077344
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA303/00/0923), Z(AV0Z50070508)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60077344:_____/06:00047087
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - N,N-dimethylformamide; globin; adducts (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Šimek, Petr
- Cimlová, Jana
- Mráz, J.
- Nohová, H.
- Stránský, V.
- Kičová, R.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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