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| Description
| - Cílem této studie bylo vyvinout metodu zmražování (spermatu) použitelnou pro uchování genofondu lína v %22ex situ%22. Úspěšnost kryokonzervace byla testována ve dvou sériích experimentů. V první byl testován efekt dvou kryoprotektantů (DMSO a kombinace DMSO s propandiolem v poměru 1:1) koncentrace 8 a 10% v kombinaci se třemi časy ekvilibrace před mražením (0.0, 2.0 a 4.0 h po T0) ve dvou různých imobilizačních roztocích (Kurokura 180 a Kurokura). K mražení 1ml vzorků byl použit program K4 a kryotuby 1,8ml. Hlavním sladovaným parametrem byla líhnivost plůdku po použití zmraženého spermatu. Ve druhé sérii experimentů byl sledován vliv objemu pejet (0,5, 1 a 5 ml) v porovnání s kryotubami 1,8 ml a vliv použitého zmrazovacího programu (K4 a L1). Na základě výsledků z první série experimentů byla použita kombinace DMSO a propandiolu (v poměru 1:1) koncentrace 10% a sperma odebrané do imobilizačního roztoku Kurokura 180. Hlavním sledovaným parametrem byla líhnivost plůdku po použití zmraženého spe (cs)
- The aim of the present study was to elaborate cryopreservation methods for ex situ conservation of tench. Success of cryopreservation was tested during two series of experiments. The first set of experiments studied the effects of two types of cryoprotectants (DMSO and a combination of DMSO with propanediol at ratio 1:1) at concentrations of 8 and 10% and three different equilibration times in two different immobilization solutions (IS) (Kurokura 180 and Kurokura) before freezing (0.0, 2.0 and 4.0 h after T0). The K4 cooling programme was used to freeze 1 ml of cryoextended sperm using 1.8 ml cryotubes. Main monitored parameter was hatching rate after using of cryopreserved sperm. The second set of experiments studied the volume effect of 0.5, 1 and 5 ml straws and compared these with 1.8 ml cryotubes as well as the effect of the cooling programme (K4 and L1). Following the results of the first study, a combination of DMSO and propanediol (ratio 1:1) at concentration of 10% was added to extended spe
- The aim of the present study was to elaborate cryopreservation methods for ex situ conservation of tench. Success of cryopreservation was tested during two series of experiments. The first set of experiments studied the effects of two types of cryoprotectants (DMSO and a combination of DMSO with propanediol at ratio 1:1) at concentrations of 8 and 10% and three different equilibration times in two different immobilization solutions (IS) (Kurokura 180 and Kurokura) before freezing (0.0, 2.0 and 4.0 h after T0). The K4 cooling programme was used to freeze 1 ml of cryoextended sperm using 1.8 ml cryotubes. Main monitored parameter was hatching rate after using of cryopreserved sperm. The second set of experiments studied the volume effect of 0.5, 1 and 5 ml straws and compared these with 1.8 ml cryotubes as well as the effect of the cooling programme (K4 and L1). Following the results of the first study, a combination of DMSO and propanediol (ratio 1:1) at concentration of 10% was added to extended spe (en)
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| Title
| - Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos
- Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos (en)
- Kryokonzervace spermatu lína obecného Tinca tinca : motilita spermií a líhnivost plůdku (cs)
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| skos:prefLabel
| - Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos
- Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos (en)
- Kryokonzervace spermatu lína obecného Tinca tinca : motilita spermií a líhnivost plůdku (cs)
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| skos:notation
| - RIV/60076658:12610/07:00007821!RIV08-MSM-12610___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA524/06/0817), Z(MSM6007665809)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/60076658:12610/07:00007821
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Cryopreservation; Hatching rate; Tench Tinca tinca; Sperm motility (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Alavi, Sayyed Mohammad Hadi
- Gela, David
- Hulák, Martin
- Linhart, Otomar
- Rodina, Marek
- Kocour, Martin
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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