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| Description
| - The proposed project will deal with spermatozoa ultrastructure, energetic and competition in two groups of model species of chondrostean (Chondrostei) and teleostean (Teleostei) fish. Study on the spermatozoa ultramorphology and ultrastructure in fish, specially focused on structure of axoneme and midpiece, to better understand the roles of mitochondria and basal body structures. This also involves studies of micromechanics and micro hydrodynamics of integrated machinery of axoneme with its associated membrane. Factors reviving motility and fertilizability in %22exhausted%22 spermatozoa of intact and demembranated spermatozoa of fish species. We anticipate the determination of activation/inhibition factors in both fish groups and firstly demonstrate that spermatozoa are able or unable fertilize the eggs with assessment of acrosome integrity. Energy requirement of machinery during spermatozoa motility for understanding why duration of motility is so short with evaluation of ATP level. Investigate the
- Předložený projekt se soustřeďuje na řešení ultrasturktury, energie a kompetice spermií u dvou modelových druhů ryb, zahrnující chrupavčité (Chondrostei) a kostnaté (Teleostei) druhy. Studie ultramorfologie a ultrastruktury spermií se zaměří na objasněnípodstaty strukturu bičíku a jeho střední části k pochopení významu struktury mitochondrií a basální části spermií. Znalosti budou využity při studiu mikromechaniky a mikrohydrodynamiky integrovaného mechanismu bičíku a membrány. Dále bude sledován faktorznovuobnovení motility a fertility u tzv. %22vyčerpaných%22 intaktních a demebranovaných spermií. U spermií ryb s obnoveným energetickým potenciál bude zdokumentována schopnnost inhibičních/aktivačních faktorů a opětovného oplození jikry. Mechanismus průnikuspermie bude u chrupavčitých ryb doplněn o studiem akrozomové integrity. Dále bude sledován energetický požadavek ATP v mechanismu motility spermií s orientací na znalost, proč doba motility rybích spermií je tak krátká. Dale se soustředíme na implikaci (en)
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| Title
| - Ultrastructure, Energetic and Competition in Spermatozoa: A Comparative Study Using Two Model Species of Chondrostean and Teleostean Fishes (en)
- Ultrastruktura, energie a kompetice spermií: Komparativní studie s využitím dvou modelových druhů chrupavčitých a kostnatých ryb
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - fish; sperm; reproduction; carp; sturgeon (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - For the project with spermatozoa ultrastructure, energetic and competition were used two groups of model species of chondrostean (Chondrostei) and teleostean (Teleostei) fish. Study on the spermatozoa ultramorphology and ultrastructure in fish, specially (en)
- Předložený projekt řešil problematiku ultrasturktury, energie a kompetice spermií u dvou modelových druhů ryb, zahrnující chrupavčité (Chondrostei) a kostnaté (Teleostei) druhy. Studie ultramorfologie a ultrastruktury spermií se zaměřila na objasnění pod (cs)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - fish
- carp
- reproduction
- sperm
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| is http://linked.open...vavai/riv/projekt
of | - After finishing of motility, common carp (Cyprinus carpio) sperm is able to re-initiate a second motility period and to fertilize eggs
- Effects of osmolality on sperm morphology, motility and flagellar wave parameters in Northen pike (Esox lucius L.)
- Structural abnormalities of common carp Cyprinus carpio spermatozoa
- Morfologie spermií, složení seminální plazmy, motilita a parametry zvlnění bičíku spermie u štiky obecné (Esox lucius)
- Does triploidization produce functional sterility of triploid males of tench Tinca tinca (L.)?
- Changes of sperm morphology, volume, density and motility and seminal plasma composition in Barbus barbus (Teleostei: Cyprinidae) during the reproductive season
- Acrosome staining and motility characteristics of sterlet spermatozoa after cryopreservation with use of methanol and DMSO
- Staining of sturgeon spermatozoa with trypsin inhibitor from soybean, Alexa Fluor? 488 conjugate for visualization of sturgeon acrosome
- Morphology and fine structure of Barbus barbus (Teleostei: Cyprinidae) spermatozoa
- Sperm motility and fertilizing ability of frozen spermatozoa of males (XY) and neomales (XX) of perch (Perca fluviatilis)
- Comparison of sperm velocity, motility and fertilizing ability between firstly and secondly activated spermatozoa of common carp (Cyprinus carpio)
- Characteristics of stripped and testicular Northern pike (Esox lucius) sperm: spermatozoa motility and velocity
- Evaluation of semen and urine of pike (Esox lucius L.): Ionic compositions and osmolality of the seminal plasma and sperm volume, density and motility
- Activation of sperm motility in the euryhaline tilapia Sarotherodon melanotheron heudelotii (Dumeril, 1859) acclimatized to fresh, sea and hypersaline waters
- Physiology and behavior of stripped and testicular sperm in Perca fluviatilis L. 1758
- A comparative study on biological aspect of sperm in sterlet and Siberian sturgeon
- Sperm characteristic in Barbus barbus as a function of nutrition throughout the reproductive season
- Cryopreservation of sperm of testicular neomales and stripped normal males of European perch (Perca fluviatilis L.).
- Determinism of the quality of reproduction in Eurasian perch, Perca fluviatilis: a multifactorial study
- A proposal and case study towards a conceptual approach of validating sperm competition in common carp (Cyprinus carpio L.), with practical implications for hatchery procedures
- Metodika hodnocení integrity akrozomu spermií jeseterů
- Monitoring of the acrosomal reaction in sturgeon using fluorescence and electron microscopy
- Morphology, chemical contents and physiology of chondrostean fish sperm: a comparative study between Siberian sturgeon (Acipenser baerii) and sterlet (Acipenser ruthenus)
- The optimization of the protocol for immunofluorescence on fish spermatozoa
- Fine structure and morphology of sterlet (Acipenser ruthenus L. 1758) spermatozoa and acrosin localization
- Monoclonal antibody FsC-47 against carp sperm creatine kinase
- Ultrastructure and morphology of spermatozoa in Chinese sturgeon (Acipenser sinensis Gray 1835) using scanning and transmission elektron microscopy
- Fish Spermatology
- Semen of Perca fluviatilis L: Sperm volume and density, seminal plasma indices and effects of dilution ratio, ions and osmolality on sperm motility
- Cryopreservation of tench, Tinca tinca, sperm: Sperm motility and hatching success of embryos
- Equalizing sperm concentrations in a common carp sperm pool does not affect variance in proportions of larvae sired in competition
- Imobilizing and cryopreservation of tench (Tinca tinca) sperm
- Morphology and ultrastructure of Siberian sturgeon (Acipenser baerii) spermatozoa using scanning and transmission electron microscopy
- Ultrastruktura spermií chrupavčitých ryb
- Quantity, motility and fertility of tench, Tinca tinca (L.) sperm in relation to LHRH analogue and carp pituitary treatments
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| is http://linked.open...vavai/cep/projekt
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