| Attributes | Values |
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| rdf:type
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| rdfs:seeAlso
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| Description
| - Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co-factor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF3 ) on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3.
- Multistep phosphorelay (MSP) signaling mediates responses to a variety of important stimuli in plants. In Arabidopsis MSP, the signal is transferred from sensor histidine kinase (HK) via histidine phosphotransfer proteins (AHP1–AHP5) to nuclear response regulators. In contrast to ancestral two-component signaling in bacteria, protein interactions in plant MSP are supposed to be rather nonspecific. Here, we show that the C-terminal receiver domain of HK CKI1 (CKI1RD) is responsible for the recognition of CKI1 downstream signaling partners, and specifically interacts with AHP2, AHP3 and AHP5 with different affinities. We studied the effects of Mg2+, the co-factor necessary for signal transduction via MSP, and phosphorylation-mimicking BeF3 ) on CKI1RD in solution, and determined the crystal structure of free CKI1RD and CKI1RD in a complex with Mg2+. We found that the structure of CKI1RD shares similarities with the only known structure of plant HK, ETR1RD, with the main differences being in loop L3. (en)
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| Title
| - Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.
- Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. (en)
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| skos:prefLabel
| - Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana.
- Structure and binding specificity of the receiver domain of sensor histidine kinase CKI1 from Arabidopsis thaliana. (en)
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| skos:notation
| - RIV/00216224:14740/11:00050106!RIV12-GA0-14740___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ED1.1.00/02.0068), P(GA521/09/1699), P(GAP305/11/0756), P(GD204/08/H054), P(LC06034), S, Z(MSM0021622413), Z(MSM0021622415)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
| - Hejátko, Jan
- Janda, Lubomír
- Dopitová, Radka
- Sklenář, Vladimír
- Žídek, Lukáš
- Papoušková, Veronika
- Horák, Jakub
- Marek, Jaromír
- Pekárová, Blanka
- Borkovcová, Petra
- Klumpler, Tomáš
- Třísková, Olga
- Nejedlá, Eliška
- Jansen, Séverine
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/00216224:14740/11:00050106
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - multistep phosphorelay; receiver domain; CKI1; crystal structure; NMR spectroscopy; Arabidopsis thaliana (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dopitová, Radka
- Hejátko, Jan
- Horák, Jakub
- Janda, Lubomír
- Marek, Jaromír
- Papoušková, Veronika
- Sklenář, Vladimír
- Žídek, Lukáš
- Pekárová, Blanka
- Borkovcová, Petra
- Jansen, Séverine
- Klumpler, Tomáš
- Nejedlá, Eliška
- Třísková, Olga
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
| |
| http://bibframe.org/vocab/doi
| - 10.1111/j.1365-313X.2011.04637.x
|
| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
of | |
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