About: Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium

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About: Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Modifications of natural DNA by the dinuclear platinum(II) organometallic complex [{Pt(Me)Cl(Me2SO)}2(-N-N)] [where N-N=H2N(CH2)6NH2] (ORGANObisPt) were studied by methods of molecular biophysics. These methods include DNA binding studies using atomic absorption spectrophotometry, HPLC analysis of enzymatically digested DNA, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, mapping of DNA adducts by transcription assay, DNA melting curves measured by absorption spectrophotometry and conformational analysis of platinated DNA by differential pulse polarography. The results indicate that the complex ORGANObisPt binds irreversibly to DNA. Its DNA binding mode is, however, different from that of the formally equivalent [{cis-PtCl(NH3)2}2(-N-N)] (1,1/c,c), which exhibits antitumor activity including that in the tumor cells resistant to cisplatin. Modifications of natural DNA by the dinuclear platinum(II) organometallic complex [{Pt(Me)Cl(Me2SO)}2(-N-N)] [where N-N=H2N(CH2)6NH2] (ORGANObisPt) were studied by methods of molecular biophysics. These methods include DNA binding studies using atomic absorption spectrophotometry, HPLC analysis of enzymatically digested DNA, interstrand cross-linking employing gel electrophoresis under denaturing conditions, DNA unwinding studied by gel electrophoresis, mapping of DNA adducts by transcription assay, DNA melting curves measured by absorption spectrophotometry and conformational analysis of platinated DNA by differential pulse polarography. The results indicate that the complex ORGANObisPt binds irreversibly to DNA. Its DNA binding mode is, however, different from that of the formally equivalent [{cis-PtCl(NH3)2}2(-N-N)] (1,1/c,c), which exhibits antitumor activity including that in the tumor cells resistant to cisplatin. (en)
Title	Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium (en)
skos:prefLabel	Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium Biophysical analysis of natural, double-helical DNA modified by a dinuclear platinum(II) organometallic compound in a cell-free medium (en)
skos:notation	RIV/00216224:14310/02:00028886!RIV10-GA0-14310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/00/0556), P(GA305/02/1552), P(IAA5004101), P(NL6058), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Brabec, Viktor Kašpárková, Jana Marini Palomeque, María Victoria
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	639728
http://linked.open...ai/riv/idVysledku	RIV/00216224:14310/02:00028886
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA; Organoplatinum(II); Adducts; Cross-link; Conformation (en)
http://linked.open.../riv/klicoveSlovo	Conformation DNA Adducts Cross-link Organoplatinum(II)
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[530A023C762A]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Inorganic Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Binuclear metal complexes as DNA-protein cross-linking agents Platinated oligonucleotides for selective modulation of gene expression; relations to antisense strategy and development of new drugs. Structure, recognition and biochemistry of DNA modified by antitumor platinum drugs Recognition and repair of DNA damage by antitumor platinum drugs.Relations to the development of new anticancer drugs.
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	7
http://linked.open...iv/tvurceVysledku	Brabec, Viktor Kašpárková, Jana Marini Palomeque, María Victoria Monsu Scolaro, Luigi Novakova, Olga Romeo, Raffaello
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0949-8257
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	14310

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