About: Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D.

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About: Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D. Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Spirochetal genus Treponema includes several closely related pathogenic spirochetes. Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis, T. pallidum ssp. pertenue (TPE) causes yaws. Although these two subspecies are microscopically and serologically indistinguishable, they cause different diseases. Little is known about genome differences between TPA and TPE genomes. The aim of this work was to employ dideoxyterminator sequencing for sequence differences discovery between Nichols (TPA) and Samoa D (TPE) genomes. All heterologous regions were successfully subjected to DDT sequencing and consensus sequences were obtained using three different approaches: 1) direct PCR product sequencing, 2) primer walking and 3) small insert library sequencing. Identified sequence differences will be used together with CGS data to compile the complete genome sequence of Samoa D strain. Spirochetal genus Treponema includes several closely related pathogenic spirochetes. Treponema pallidum ssp. pallidum (TPA) is the causative agent of syphilis, T. pallidum ssp. pertenue (TPE) causes yaws. Although these two subspecies are microscopically and serologically indistinguishable, they cause different diseases. Little is known about genome differences between TPA and TPE genomes. The aim of this work was to employ dideoxyterminator sequencing for sequence differences discovery between Nichols (TPA) and Samoa D (TPE) genomes. All heterologous regions were successfully subjected to DDT sequencing and consensus sequences were obtained using three different approaches: 1) direct PCR product sequencing, 2) primer walking and 3) small insert library sequencing. Identified sequence differences will be used together with CGS data to compile the complete genome sequence of Samoa D strain. (en)
Title	Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D. Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D. (en)
skos:prefLabel	Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D. Comparison ot the syphilis and yaws spirochete genomes: comparative genome sequencing of T. pallidum ssp. pallidum Samoa D. (en)
skos:notation	RIV/00216224:14110/06:00016083!RIV10-MZ0-14110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/04/0021), P(NR8967), Z(MSM0021622415)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Šmajs, David Matějková, Petra
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Masarykova univerzita / Lékařská fakulta
http://linked.open...dnocenehoVysledku	469344
http://linked.open...ai/riv/idVysledku	RIV/00216224:14110/06:00016083
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	comparative genome sequencing; T. pallidum ssp. pallidum Samoa D (en)
http://linked.open.../riv/klicoveSlovo	T. pallidum ssp. pallidum Samoa D comparative genome sequencing
http://linked.open...ontrolniKodProRIV	[C1107B1731C1]
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Postgenomic studies on Treponema pallidum, the syphilis spirochete Molecular typing of Treponema pallidum subsp. pallidum strains and isolates: the background for epidemiological and clinical identifications of individual strains
http://linked.open...UplatneniVysledku	2006
http://linked.open...iv/tvurceVysledku	Pospíšilová, Petra Šmajs, David
http://linked.open...n/vavai/riv/zamer	Molecular basis of cell and tissue regulations
http://localhost/t...ganizacniJednotka	14110

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