About: Methodology of analytical electrophoresis of samples with complex matrices

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rdf:type	http://linked.opendata.cz/ontology/domain/vavai/Projekt
rdfs:seeAlso	http://www.isvav.cz/projectDetail.do?rowId=IAA4031703
Description	The objective of the grant project is the development of new principles and procedures for analyses of samples with complex biochemical matrices by capillary electrophoresis. The research will be aimed at investigating and developing new on.line preconcentration, preseparation and sample clean-up procedures. The main principles which will be utilized are electrophoretic stacking techniques with sophisticated instrumentation and configuration of electrolytes, selective solid and liquid phase extraction and electroelution, sieving effects at liquid-solid and liquid-liquid interfaces, selective micellar solubilization and charge based clean-up. (en) Předmětem grantového návrhu je vývoj nových principů a postupů pro analýzy vzorků se složitými biochemickými matricemi kapilární elektroforézou. Výzkum bude zaměřen na vývoj nových on-line předkoncentračních a předseparačních postupů. Hlavními principy, které budou využity, jsou elektroforetické koncentrační techniky spojené s vyspělou instrumentací i volbou pracovních elektrolytů, selektivní extrakce a elektroeluce z pevné a kaplané fáze, sítové efekty na rozhraních kapalina-pevná fáze a kapalina-kapalina, selektivní micelární solubilizace a nábojově podmíněná předseparace vzorku.
Title	Methodology of analytical electrophoresis of samples with complex matrices (en) Metodologie analytické elektroforézy vzorků se složitými matricemi
skos:notation	IAA4031703
http://linked.open...avai/cep/aktivita	Grants of distinctly investigative character focused on the sphere of research pursued at present particularly in the Academy of Sciences of the Czech Republic
http://linked.open...kovaStatniPodpora	http://linked.opendata.cz/resource/domain/vavai/projekt/IAA4031703/celkovaStatniPodpora
http://linked.open...ep/celkoveNaklady	http://linked.opendata.cz/resource/domain/vavai/projekt/IAA4031703/celkoveNaklady
http://linked.open...datumDodatniDoRIV	2007-01-15 (xsd:date)
http://linked.open...i/cep/druhSouteze	VS - Veřejná soutěž ve výzkumu a vývoji
http://linked.open...ep/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open.../cep/fazeProjektu	767635
http://linked.open...ai/cep/hlavniObor	CB - Analytická chemie, separace
http://linked.open...hodnoceniProjektu	V - Vynikající výsledky (s mezinárodním významem apod.). Zároveň byly splněny cíle a předpokládané výsledky uvedené ve smlouvě / rozhodnutí o poskytnutí podpory.
http://linked.open.../cep/klicovaSlova	capillary electrophoresis; trace analysis; methodology of electrophoresis; (en)
http://linked.open...ep/partnetrHlavni	Ústav analytické chemie AV ČR, v. v. i.
http://linked.open...inujicichPrijemcu	0 (xsd:int)
http://linked.open...cep/pocetPrijemcu	1 (xsd:int)
http://linked.open...ocetSpoluPrijemcu	0 (xsd:int)
http://linked.open.../pocetVysledkuRIV	51 (xsd:int)
http://linked.open...enychVysledkuVRIV	51 (xsd:int)
http://linked.open...iciPoslednihoRoku	2002
http://linked.open...atUdajeProjZameru	2002
http://linked.open...usZobrazovaneFaze	DUU
http://linked.open...ai/cep/typPojektu	P - Projekt výzkumu a vývoje financovaný ze státního rozpočtu
http://linked.open...jektu+dodavatelem	Byly vyvinuty nové principy a postupy pro stopové analýzy biologických vzorků se složitou matricí. Na zákl. teor. rozborů a experiment. studií byly vypracovány zásady pro optimalizaci separačních podmínek a maximalizaci citlivosti analýz. (cs)
http://linked.open...tniCyklusProjektu	ZBBBKU
http://linked.open.../cep/klicoveSlovo	capillary electrophoresis trace analysis methodology of electrophoresis
is http://linked.open...vavai/riv/projekt of	Fast separation of DNA sequencing fragments in highly alkaline solutions of linear polyacrylamide using electrophoresis in bare silica capillaries. Electrophoretic behaviour and properties of sanguinarine and chelerythrine. Exploration of the electrophoretic behaviour of borane cluster anions and of the capability of capillary electrophoresis to separate them chirally. N/A Continuous free-flow electrophoresis. Quantitative trace analysis of L-ascorbic acid in human body fluids by on-line combination of capillary isotachophoresis and zone electrophoresis. Predicting peak symmetry in capillary zone electrophoresis. Background electrolytes with two co-ions: schizophrenic zone broadening and the role of system peaks. 'Schizophrenic' behavior of zones in capillary zone electrophoresis: Explanation of an old problem. Chiral separations in capillary electrophoresis. Isotachophoresis in zone electrophoresis. The effect of sample composition on course of separation by capillary zone electrophoresis. Capillary zone electrophoresis and micellar electrokinetic chromatography of solution of polyaniline particles. Calculation of stability constants for the chiral selector-enantiomer interactions from electrophoretic mobilities. BGE systems: characteristics and performance. BGE systems in CZE. Characteristics and performance of multivalent electrolyte systems. Chiral separations in capillary electrophoresis. Capillary zone electrophoresis in phosphate buffer - known or unknown? New aspects of buffering with multivalent weak acids in capillary zone electrophoresis: Pros and cons of the phosphate buffer. Fast searching of optimum separation conditions for analyses with the method of capillary zone electrophoresis using informatic system 'Xemic'. Quantitation of trace analytes in capillary zone electrophoresis with UV-absorbance detection: A critical study of approaches based on peak height. Electrophoretic investigation of interactions of sanguinarine and chelerythrine with molecules containing mercapto group Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries Transient isotachophoresis as a tool for enhancement of sensitivity in CZE analyses of samples with complex matrices. Achiral separations of deltahedral borate anions by capillary zone electrophoresis. System zones in capillary zone electrophoresis Success and fiasco with robust electrolyte systems in CZE. Study of electrophoretic properties of sanguinarine and chelerythrine. Success and failure with phthalate buffers in capillary zone electrophoresis. Why robust background electrolytes containing multivalent ionic species can fail in capillary zone electrophoresis. Multivalent weak electrolytes - risky background electrolytes for capillary zone electrophoresis. Multiple effect of surfactants used as additives in background electrolytes in capillary zone electrophoresis: Cetyltrimethylammonium bromide as example of model surfactant. Fast detection of a (CA)(18) microsatellite repeat in the IgE receptor gene by capillary electrophoresis with laser induced fluorescence detection Interval isotachophoresis for purification and isolation of ionogenic species. Stability of continuous flow electrophoresis. A new electrode chamber for stable performance in capillary electrophoresis. Analysis of orotic acid in human urine by on-line combination of capillary isotachophoresis and zone electrophoresis. Analytical expediency of acidic and alkaline background electrolytes in capillary zone electrophoresis: role of system zones in single-co-ion systems. Experimental technique for simultaneous adsorption and streaming current studies: liquid chromatography - streaming current hyphenation. Peaks in capillary electrophoresis: Fact or fiction. Determination of dissociation constants of cytokinins by capillary zone electrophoresis. Sample stacking in capillary zone electrophoresis: Principles, advantages and limitations. Selectivity in capillary electrophoresis. Sample self-stacking in capillary zone electrophoresis: Behavior of samples containing multiple major coionic components. Chloride present in biological samples as a tool for enhancement of sensitivity in CZE analysis of trace analytes. Chiral separations of charged boranes. Chiral separations of charged boranes by capillary zone electrophoresis. Chloride present in biological samples as a tool for enhancement of sensitivity in CZE analysis of trace analytes. Impact of sample matrix on quantitation in CZE. Mechanism of streaming current generation by concentration gradients in packed-particle columns. Interactions of sanguinarine and chelerythrine with molecules containing a mercapto group. The preparation of background electrolytes in capillary zone electrophoresis: Golden rules and pitfalls.
is http://linked.open...vavai/cep/projekt of	http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA4031703/1997/ico%3A68081715 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA4031703/1998/ico%3A68081715 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA4031703/1999/ico%3A68081715 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA4031703/2000/ico%3A68081715 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA4031703/2001/ico%3A68081715 http://linked.opendata.cz/resource/domain/vavai/cep/ucast/IAA4031703/2002/ico%3A68081715

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