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| Description
| - The project will deal with the synthesis of novel types of conjugates of purine and pyrimidine bases with metal complexes (e.g. ferrocene, transition metal complexes of bipyridine or phenanthroline etc.) connected by a conjugated linker. Such labelled nucleosides will be transformed to protected phosphoramidites and use in oligonucleotide synthesis. We will study electrochemical behaviour of the labelled oligonucleotides in their single-stranded as well as double-stranded forms (including duplexes involving single-base mismatches). The goal is to find a system whose electrochemical features will be significantly affected by the duplex formation. Such system will be utilized in development of sensors for DNA hybridization. We suppose that effects of interactions between base pairs in the DNA double helix will be transferred through the conjugated system to the marker (metal complex). Potential markers will be also studied by theoretical chemistry calculations and the calculated ionization (en)
- Projekt bude zaměřen na syntézu nových typů konjugátů purinových a pyrimidinových bází a nukleosidů s komplexy kovů (např. ferrocen, komplexy bipyridinu či fenanthrolinu s různými přechodnými kovy atd.) spojených přes konjugovaný linker. Takto označené nukleosidy budou převedeny na chráněné fosforamidity a použity v syntéze oligonukleotidů. Bude studováno elektrochemické chování značených oligonukletidů v jejich jednořetězcové a dvouřetězcové formě (včetně duplexů s řetězci obsahujícími bodové mutace).Cílem bude najít systém, jehož elektrochemické vlastnosti budou výrazně ovlivněny tvorbou duplexu DNA, a využít jej při vývoji senzorů pro hybridizaci DNA. Předpokládáme, že se vliv interakcí mezi páry bazí v dvoušroubovici DNA přenese přes konjugovaný systém na marker (komplex kovu). Potenciální markery budou také studovány teoretickými výpočetními metodami a vypočtené ionizační potenciály srovnávány s experimentálními daty. Výpočty budou využity při racionálním designu nových markerů.
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| Title
| - Conjugates of nucleobases with metal complexes as electroactive markers. Application of labelled oligonucleotides in electrochemical DNA sensors (en)
- Konjugáty nukleobází s komplexy kovů jako elektroaktivní značky. Využití značených oligonukleotidů v senzorech pro hybridizaci DNA
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| skos:notation
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| http://linked.open...avai/cep/aktivita
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| http://linked.open...kovaStatniPodpora
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| http://linked.open...ep/celkoveNaklady
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| http://linked.open...datumDodatniDoRIV
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| http://linked.open...i/cep/druhSouteze
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| http://linked.open...ep/duvernostUdaju
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| http://linked.open.../cep/fazeProjektu
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| http://linked.open...ai/cep/hlavniObor
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| http://linked.open...hodnoceniProjektu
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| http://linked.open...vai/cep/kategorie
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| http://linked.open.../cep/klicovaSlova
| - nucleobases; nucleotides; oligonucleotides; metal complexes; electrochemistry; sensors; DNA (en)
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| http://linked.open...ep/partnetrHlavni
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| http://linked.open...inujicichPrijemcu
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| http://linked.open...cep/pocetPrijemcu
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| http://linked.open...ocetSpoluPrijemcu
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| http://linked.open.../pocetVysledkuRIV
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| http://linked.open...enychVysledkuVRIV
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| http://linked.open...lneniVMinulemRoce
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| http://linked.open.../prideleniPodpory
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| http://linked.open...iciPoslednihoRoku
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| http://linked.open...atUdajeProjZameru
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| http://linked.open.../vavai/cep/soutez
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| http://linked.open...usZobrazovaneFaze
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| http://linked.open...ai/cep/typPojektu
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| http://linked.open...ep/ukonceniReseni
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| http://linked.open.../cep/vedlejsiObor
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| http://linked.open...ep/zahajeniReseni
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| http://linked.open...jektu+dodavatelem
| - Cíle projektu byly splněny a značně rozšířeny. Byla vypracována metodika arylací a alkynylací nukleobází a nukleosidů cross-coupling reakcemi a syntéza konjugátů nukleobází a nukleosidů s Ru a Os komplexy. Původní záměr připravovat značené oligonukleotid (cs)
- The goals of the project have been fulfilled and significantly extended. Methodology for arylation and alkynylation of nucleobases and nucleosides by cross-coupling reactions and synthesis of conjugates of nucleobases and nucleosides with Ru and Os compl (en)
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| http://linked.open...tniCyklusProjektu
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| http://linked.open.../cep/klicoveSlovo
| - nucleobases
- electrochemistry
- metal complexes
- nucleotides
- oligonucleotides
- sensors
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| is http://linked.open...vavai/riv/projekt
of | - Aminophenyl- and nitrophenyl-labeled nucleoside triphosphates. Synthesis, enzymatic incorporation and electrochemical detection
- Synthesis of modified nucleosides. Nucleotides and oligonucleotides bearing metal complexes
- Direct C-H arylation of purines and purine nucleosides
- Theoretical study of the stability of the DNA duplexes modified by a series of hydrophobic base analogues
- Cross-coupling reactions of nucleoside triphosphates followed by polymerase incorporation. Construction and applications of base-functionalized nucleic acids
- Aminophenyl- and nirophenyl-labeled DNA. Synthesis by polymerase incorporation of nucleoside triphosphates and electrochemical properties
- Effect of spin-orbit coupling on reduction potentials of octahedral ruthenium (II/III) and osmium (II/III) complexes
- Exceptional Thermodynamic Stability of DNA Duplexes Modified by Nonpolar Base Analogues Is due to Increased Stacking Interactions and Favorable Solvation: Correlated Ab Initio Calculations and Molecular Dynamics Simulations
- Aqueous-phase Suzuki-Miyaura cross-coupling reactions of free halopurine bases
- Direct C-H arylation of purines: Development of methodology and its use in regioselective synthesis of 2,6,8-trisubstituted purines
- An efficient method for the construction of functionalized DNA bearing amino acid groups through cross-coupling reactions of nucleoside triphosphates followed by primer extension or PCR
- Purines bearing phenanthroline or bipyridine ligands and their Ru II complexes in position 8 as model compounds for electrochemical DNA labeling - synthesis, crystal structure, electrochemistry, quantum calculations, cytostatic and antiviral activity
- Synthesis of 2'-deoxyadenosine nucleosides bearing bipyridine-type ligands and their Ru-complexes in position 8 through cross-coupling reactions
- Ferrocenylethynyl derivatives of nucleoside triphosphates: Synthesis, incorporation, electrochemistry, and bioanalytical applications
- %22Multicolor%22 electrochemical labeling of DNA hybridization probes with osmium tetroxide complexes
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| is http://linked.open...vavai/cep/projekt
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