About: First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic

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About: First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars was surveyed in 2009. A total of 67 plants of 10 cultivars were observed for typical Blueberry red ringspot virus (BRRV) symptoms and were also assayed for BRRV infection using PCR. Extracted DNA from leaf tissue was used in PCR for BRRV detection with primer pair BRRV15/16. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16. The amplicons were sequenced and revealed 97.5% nucleotide sequence identity to the BRRV (GenBank accession number AF404509). The 845 nucleotide sequence of the amplicon has been deposited at GenBank under accession number HM107773. A collection of highbush blueberry (Vaccinium corymbosum L.) cultivars was surveyed in 2009. A total of 67 plants of 10 cultivars were observed for typical Blueberry red ringspot virus (BRRV) symptoms and were also assayed for BRRV infection using PCR. Extracted DNA from leaf tissue was used in PCR for BRRV detection with primer pair BRRV15/16. No BRRV symptoms were observed on the plants in early spring, yet BRRV was detected in one symptom-free bush of cv. Darrow by PCR. In July, typical symptoms developed on that and another cv. Darrow bush that was also positive by PCR. DNA fragments were amplified from total nucleic acid samples of both infected blueberry bushes using primers BRRV15/16. The amplicons were sequenced and revealed 97.5% nucleotide sequence identity to the BRRV (GenBank accession number AF404509). The 845 nucleotide sequence of the amplicon has been deposited at GenBank under accession number HM107773. (en)
Title	First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic (en)
skos:prefLabel	First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic First Report of Blueberry red ringspot virus in Highbush Blueberry in the Czech Republic (en)
skos:notation	RIV/60077344:_____/10:00345901!RIV13-AV0-60077344
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(OC09022), Z(AV0Z50510513)
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Přibylová, Jaroslava Petrzik, Karel Špak, Josef Kubelková, Darina
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biologické centrum AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	259311
http://linked.open...ai/riv/idVysledku	RIV/60077344:_____/10:00345901
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Virus; small fruits; pathogen detection (en)
http://linked.open.../riv/klicoveSlovo	Virus pathogen detection small fruits
http://linked.open...ontrolniKodProRIV	[205E82A2D267]
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Survey on virus and phytoplasma diseases of Vaccinium in the Czech Republic
http://linked.open...UplatneniVysledku	2010
http://linked.open...iv/tvurceVysledku	Petrzik, Karel Přibylová, Jaroslava Špak, Josef Kubelková, Darina
http://linked.open...n/vavai/riv/zamer	Výzkum struktury genetické informace rostlin a jejich patogenů na molekulární úrovni, indukce a analýza cílených změn genomu a plastomu a studium fotosyntetických procesů a projevů dědičnosti v interakci s prostředím a patogeny

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