About: Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis

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About: Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Prostate cancer with altered zinc(II) cell metabolism is the second most frequently diagnosed cancer in developed countries. The aim of this article was to optimize immunoseparation protocol based on paramagnetic beads conjugated with protein G for the isolation of metallothionein. We observed strong correlation in all experimental groups and all lysate types (r>0.83 at po0.041) between metallothionein concentration related to viability and concentration of copper(II) ions and cadmium(II) ions in medium. Prostate cancer with altered zinc(II) cell metabolism is the second most frequently diagnosed cancer in developed countries. The aim of this article was to optimize immunoseparation protocol based on paramagnetic beads conjugated with protein G for the isolation of metallothionein. We observed strong correlation in all experimental groups and all lysate types (r>0.83 at po0.041) between metallothionein concentration related to viability and concentration of copper(II) ions and cadmium(II) ions in medium. (en)
Title	Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis (en)
skos:prefLabel	Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis (en)
skos:notation	RIV/00216305:26620/11:PU95709!RIV12-MSM-26620___
http://linked.open...avai/predkladatel	Středoevropský technologický institut
http://linked.open...avai/riv/aktivita	P V
http://linked.open...avai/riv/aktivity	P(ED1.1.00/02.0068), P(GP301/09/P436), P(NS10200), V
http://linked.open...iv/cisloPeriodika	24
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Ryvolová, Markéta Křížková, Soňa Babula, Petr Adam, Vojtěch Kizek, René Sochor, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Vysoké učení technické v Brně / Středoevropský technologický institut
http://linked.open...dnocenehoVysledku	205922
http://linked.open...ai/riv/idVysledku	RIV/00216305:26620/11:PU95709
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Immunoprecipitation, Metallothionein, Microparticles, Microseparation, Prostate cancer (en)
http://linked.open.../riv/klicoveSlovo	Metallothionein Microparticles Prostate cancer Immunoprecipitation Microseparation
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[8342ECD23497]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Metallothionein analysis in prostate carcinoma at DNA, RNA and protein level. Tumor markers, their determination and correlation with prostate carcinoma
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Babula, Petr Gumulec, Jaromír Jurajda, Michal Kizek, René Křížková, Soňa Masařík, Michal Ryvolová, Markéta Sochor, Jiří Sztalmachová, Markéta Hlavna, Marian
http://linked.open...ain/vavai/riv/wos	000298587900014
issn	0173-0835
number of pages	13 (xsd:int)
http://localhost/t...ganizacniJednotka	26620
is http://linked.open...avai/riv/vysledek of	Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis Isolation of metallothionein from cells derived from aggressive form of high-grade prostate carcinoma using paramagnetic antibody-modified microbeads off-line coupled with electrochemical and electrophoretic analysis

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