About: Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://dx.doi.org/10.1007/s00792-012-0450-3
Description	We explored the use of Raman spectroscopy to detect organic osmotic solutes as biomarkers in the moderately halophilic heterotrophic bacterium grown in complex medium (accumulation of glycine betaine) and in defined medium with glucose as carbon source (biosynthesis of ectoine), and in the anoxygenic phototrophic known to synthesize glycine betaine in combination with minor amounts of trehalose and -alpha-carbamoyl glutamineamide. We tested different methods of preparation of the material: lyophilization, two-phase extraction of water-soluble molecules, and perchlorate extraction. Raman signals of glycine betaine and ectoine were detected; perchlorate extraction followed by desalting the extract on an ion retardation column gave the best results. Lyophilized cells of showed strong signals of carotenoid pigments, and glycine betaine could be detected only after perchlorate extraction and desalting. The data presented show that Raman spectroscopy is a suitable tool to assess the mode of osmotic adaptation used by halophilic microorganisms. We explored the use of Raman spectroscopy to detect organic osmotic solutes as biomarkers in the moderately halophilic heterotrophic bacterium grown in complex medium (accumulation of glycine betaine) and in defined medium with glucose as carbon source (biosynthesis of ectoine), and in the anoxygenic phototrophic known to synthesize glycine betaine in combination with minor amounts of trehalose and -alpha-carbamoyl glutamineamide. We tested different methods of preparation of the material: lyophilization, two-phase extraction of water-soluble molecules, and perchlorate extraction. Raman signals of glycine betaine and ectoine were detected; perchlorate extraction followed by desalting the extract on an ion retardation column gave the best results. Lyophilized cells of showed strong signals of carotenoid pigments, and glycine betaine could be detected only after perchlorate extraction and desalting. The data presented show that Raman spectroscopy is a suitable tool to assess the mode of osmotic adaptation used by halophilic microorganisms. (en)
Title	Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria (en)
skos:prefLabel	Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria (en)
skos:notation	RIV/00216208:11310/12:10128074!RIV13-GA0-11310___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GAP210/10/0467), Z(MSM0021620855)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Jehlička, Jan Vítek, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Karlova v Praze / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	176403
http://linked.open...ai/riv/idVysledku	RIV/00216208:11310/12:10128074
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ectothiorhodospira; Halomonas; ectoine; glycine betaine; Raman spectroscopy; Compatible solutes (en)
http://linked.open.../riv/klicoveSlovo	glycine betaine Raman spectroscopy Compatible solutes Ectothiorhodospira Halomonas ectoine
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[A36402BA2DD7]
http://linked.open...i/riv/nazevZdroje	Extremophiles : life under extreme conditions
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Detekce exobiologických markerů Ramanovou spektrometrií jako hlavní metodou
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	16
http://linked.open...iv/tvurceVysledku	Jehlička, Jan Vítek, Petr Oren, Aharon
http://linked.open...ain/vavai/riv/wos	000303870300016
http://linked.open...n/vavai/riv/zamer	Material flow mechanisms in the upper spheres of the Earth
issn	1431-0651
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00792-012-0450-3
http://localhost/t...ganizacniJednotka	11310
is http://linked.open...avai/riv/vysledek of	Use of Raman spectroscopy for identification of compatible solutes in halophilic bacteria

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