About: Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Cyprinid herpesvirus-3(CyHV-3) is a causative agent of the lethal disease in koi and common carp that can adversely affect common and koi carp breeds.At present, several different PCR tests are in use.In this study, we compared the sensitivity of the single-round and nested PCR assays by testing the panel of field tissue samples(106) of koi and common carp and by testing tissue samples with known amounts of CyHV-3 DNA. The sensitivity of all the PCR methods was similar within testing the spiked homogenate sample with a known amount of CyHV-3 DNA (ca.12 DNA copies), with the exception of Gray PCR (25 copies). Detection levels of the individual PCR methods in field samples varied.The most sensitive and suitable seems to be two-round Bercovier method, detection of 44,3% of positive samples in total. Cyprinid herpesvirus-3(CyHV-3) is a causative agent of the lethal disease in koi and common carp that can adversely affect common and koi carp breeds.At present, several different PCR tests are in use.In this study, we compared the sensitivity of the single-round and nested PCR assays by testing the panel of field tissue samples(106) of koi and common carp and by testing tissue samples with known amounts of CyHV-3 DNA. The sensitivity of all the PCR methods was similar within testing the spiked homogenate sample with a known amount of CyHV-3 DNA (ca.12 DNA copies), with the exception of Gray PCR (25 copies). Detection levels of the individual PCR methods in field samples varied.The most sensitive and suitable seems to be two-round Bercovier method, detection of 44,3% of positive samples in total. (en)
Title	Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods (en)
skos:prefLabel	Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods (en)
skos:notation	RIV/00027162:_____/10:#0000611!RIV11-MZE-00027162
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QH71057), Z(MSM6007665809), Z(MZE0002716201), Z(MZE0002716202)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Reschová, Stanislava Veselý, Tomáš Pokorová, Dagmar Hůlová, Jana Vícenová, Monika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Výzkumný ústav veterinárního lékařství, v.v.i.
http://linked.open...dnocenehoVysledku	253636
http://linked.open...ai/riv/idVysledku	RIV/00027162:_____/10:#0000611
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Koi; Common carp; polymerase chain reaction (PCR) (en)
http://linked.open.../riv/klicoveSlovo	Common carp Koi polymerase chain reaction (PCR)
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[AE39D07E5084]
http://linked.open...i/riv/nazevZdroje	Journal of the World Aquaculture Society
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Monitoring of the koi herpes virus disease (KHVD) occurrence in breeds of common carp in CR and testing of susceptibility of chosen strains of common carp to KHV.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	41
http://linked.open...iv/tvurceVysledku	Reschová, Stanislava Veselý, Tomáš Hůlová, Jana Pokorová, Dagmar Vícenová, Monika Piačková, V.
http://linked.open...ain/vavai/riv/wos	000282697900011
http://linked.open...n/vavai/riv/zamer	Biologické, enviromentální a chovatelské aspekty v rybářství Zdravá zvířata a bezpečné potraviny jako základ pro zdraví lidí Animal disease research, prevention and food chain protection
issn	0893-8849
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods Detection of Cyprinid Herpesvirus-3 in Field Samples of Common and Koi Carp by Various Single-Round and Nested PCR Methods

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