About: Native Red Electrophoresis - A new method suitable for separation of native proteins

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A new type of native electrophoresis was developed to separate and characterize proteins. In this modification of the native blue electrophoresis, the dye Ponceau Red S is used instead of Coomassie Brilliant Blue to impose uniform negative charge on proteins to enable their electrophoretic separation according to their relative molecular masses. As Ponceau Red S binds less tightly to proteins, in comparison with Coomassie Blue, it can be easily removed after the electrophoretic separation and a further investigation of protein properties is made possible (e.g. an enzyme detection or electroblotting). The tested proteins also kept their native properties (enzyme activity or aggregation state). A new type of native electrophoresis was developed to separate and characterize proteins. In this modification of the native blue electrophoresis, the dye Ponceau Red S is used instead of Coomassie Brilliant Blue to impose uniform negative charge on proteins to enable their electrophoretic separation according to their relative molecular masses. As Ponceau Red S binds less tightly to proteins, in comparison with Coomassie Blue, it can be easily removed after the electrophoretic separation and a further investigation of protein properties is made possible (e.g. an enzyme detection or electroblotting). The tested proteins also kept their native properties (enzyme activity or aggregation state). (en)
Title	Native Red Electrophoresis - A new method suitable for separation of native proteins Native Red Electrophoresis - A new method suitable for separation of native proteins (en)
skos:prefLabel	Native Red Electrophoresis - A new method suitable for separation of native proteins Native Red Electrophoresis - A new method suitable for separation of native proteins (en)
skos:notation	RIV/86652036:_____/11:00371665!RIV13-AV0-86652036
http://linked.open...avai/predkladatel	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA303/09/1285), Z(AV0Z50520701)
http://linked.open...iv/cisloPeriodika	24
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Maňásková-Postlerová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	215008
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/11:00371665
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	native electrophoresis; Ponceau RR; Ponceau S; protein (en)
http://linked.open.../riv/klicoveSlovo	Ponceau RR Ponceau S native electrophoresis protein
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[67D4A1C2D797]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Study of the sperm membrane proteins with zona pellucida binding activity
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	32
http://linked.open...iv/tvurceVysledku	Maňásková-Postlerová, Pavla Doubnerová, V. Ryšlavá, H. Dráb, T. Hanzlíková, E. Kračmerová, J. Liberda, J. Tichá, I. Tichá, M.
http://linked.open...ain/vavai/riv/wos	000298587900016
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR
issn	0173-0835
number of pages	3 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jssc.201000869
is http://linked.open...avai/riv/vysledek of	Native Red Electrophoresis - A new method suitable for separation of native proteins

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