About: Human seminal plasma proteins on sperm surface detected by monoclonal antibodies

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We have generated antibodies to sperm and in our panel of mouse monoclonal antibodies to human spermatozoa several antibodies - Hs-3, Hs-16, Hs-23, Hs-9, Hs-31, Hs-32 and Hs-37 - reacted with human seminal plasma. These antibodies were characterized in detail using indirect immunofluorescence, Western blot techniques and MALDI TOF sequence analysis and recognized apolipoprotein J (clusterin), semenogelin I, secretory actin-binding protein (SABP) and enolase I. Proteins detected by our monoclonal antibodies can be useful in investigation of the mechanisms involved in sperm pathology and also represent candidates for diagnostic markers during sperm quality assessement. Further experiments concerning the role of above mentioned proteins in capacitation, acrosomal reaction and sperm-egg binding are in progress. We have generated antibodies to sperm and in our panel of mouse monoclonal antibodies to human spermatozoa several antibodies - Hs-3, Hs-16, Hs-23, Hs-9, Hs-31, Hs-32 and Hs-37 - reacted with human seminal plasma. These antibodies were characterized in detail using indirect immunofluorescence, Western blot techniques and MALDI TOF sequence analysis and recognized apolipoprotein J (clusterin), semenogelin I, secretory actin-binding protein (SABP) and enolase I. Proteins detected by our monoclonal antibodies can be useful in investigation of the mechanisms involved in sperm pathology and also represent candidates for diagnostic markers during sperm quality assessement. Further experiments concerning the role of above mentioned proteins in capacitation, acrosomal reaction and sperm-egg binding are in progress. (en)
Title	Human seminal plasma proteins on sperm surface detected by monoclonal antibodies Human seminal plasma proteins on sperm surface detected by monoclonal antibodies (en)
skos:prefLabel	Human seminal plasma proteins on sperm surface detected by monoclonal antibodies Human seminal plasma proteins on sperm surface detected by monoclonal antibodies (en)
skos:notation	RIV/86652036:_____/09:00326897!RIV10-MSM-86652036
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06011), Z(AV0Z50520701)
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pěknicová, Jana Elzeinová, Fatima Margaryan, Hasmik Čapková, Jana Koubek, Pavel
http://linked.open.../riv/druhVysledku	O - Ostatní výsledky nezařaditelné do žádného z výše uvedených druhů výsledku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biotechnologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	318034
http://linked.open...ai/riv/idVysledku	RIV/86652036:_____/09:00326897
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	monoclonal antibody; MALDI/TOF; human seminal plasma; sperm surface proteins (en)
http://linked.open.../riv/klicoveSlovo	sperm surface proteins human seminal plasma monoclonal antibody MALDI/TOF
http://linked.open...ontrolniKodProRIV	[48A653ACA19E]
http://linked.open...in/vavai/riv/obor	DN
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The Centre of Molecular Methods for Monitoring the Diffuse Pollution of the Environment.
http://linked.open...UplatneniVysledku	2009
http://linked.open...iv/tvurceVysledku	Elzeinová, Fatima Pěknicová, Jana Margaryan, Hasmik Čapková, Jana Koubek, Pavel
http://linked.open...n/vavai/riv/zamer	Establishing of the Biotechnology Institute ASCR

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