About: In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	DNA polymerase (pol) ϵ is thought to be the leading strand replicase in eukaryotes, whereas pols λ and β are believed to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol ϵ is halted by an abasic site (apurinic/apyrimidinic (AP) site). In this study, we present in vitro evidence that human pols λ, β, and η can perform translesion synthesis (TLS) of an AP site in the presence of pol ϵ, likely by initiating the 3OHs created at the lesion by the arrested pol ϵ. However, in the case of pols λ and β, this TLS requires the presence of a DNA gap downstream from the product synthesized by the pol ϵ, and the optimal gap for efficient TLS is different for the two polymerases. Collectively, our in vitro results support the existence of a mechanism of gap-directed TLS at an AP site involving a switch between the replicative pol ϵ and the repair pols λ and β. DNA polymerase (pol) ϵ is thought to be the leading strand replicase in eukaryotes, whereas pols λ and β are believed to be mainly involved in re-synthesis steps of DNA repair. DNA elongation by the human pol ϵ is halted by an abasic site (apurinic/apyrimidinic (AP) site). In this study, we present in vitro evidence that human pols λ, β, and η can perform translesion synthesis (TLS) of an AP site in the presence of pol ϵ, likely by initiating the 3OHs created at the lesion by the arrested pol ϵ. However, in the case of pols λ and β, this TLS requires the presence of a DNA gap downstream from the product synthesized by the pol ϵ, and the optimal gap for efficient TLS is different for the two polymerases. Collectively, our in vitro results support the existence of a mechanism of gap-directed TLS at an AP site involving a switch between the replicative pol ϵ and the repair pols λ and β. (en)
Title	In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta (en)
skos:prefLabel	In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta (en)
skos:notation	RIV/68378050:_____/11:00372429!RIV12-AV0-68378050
http://linked.open...avai/predkladatel	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	37
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Shevelev, Igor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	204041
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/11:00372429
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA damage; DNA polymerase; DNA repair; DNA replication; DNA-protein interaction (en)
http://linked.open.../riv/klicoveSlovo	DNA polymerase DNA-protein interaction DNA damage DNA replication DNA repair
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[D66EF6A60C10]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	286
http://linked.open...iv/tvurceVysledku	Gironis, N. Hübscher, U. Le Gac, N.T. Markennen, E. Parkkinen, S. Pospiech, H. Shevelev, Igor Syvaaja, J. E. Villani, G. di Cicco, G.
http://linked.open...ain/vavai/riv/wos	000294726800019
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0021-9258
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1074/jbc.M111.246611
is http://linked.open...avai/riv/vysledek of	In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta In vitro gap-directed translesion DNA synthesis of an abasic site involving human DNA polymerases epsilon, lambda, and beta

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