About: Clonal analysis of hematopoietic progenitor cells in the zebrafish

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis. Identification of hematopoietic progenitor cells in the zebrafish has been hindered by a lack of functional assays to gauge proliferative potential and differentiation capacity. To investigate the nature of myeloerythroid progenitor cells, we developed clonal methylcellulose assays utilizing recombinant zebrafish erythropoietin (Epo) and granulocyte colony stimulating factor (Gcsf). Epo was required to support erythroid colony formation, and Gcsf to support the formation of myeloid colonies. Colonies showed distinct morphologies and were easily scored by their expression of lineage-specific fluorescent transgenes. The majority of progenitor activity was contained within the precursor light scatter fraction, and more immature precursors were present within the lymphoid fraction. These studies provide the first report of clonal hematopoietic progenitor assays in the zebrafish, and establish the number, characteristics, and kinetics of myeloerythroid progenitors during hematopoiesis. (en)
Title	Clonal analysis of hematopoietic progenitor cells in the zebrafish Clonal analysis of hematopoietic progenitor cells in the zebrafish (en)
skos:prefLabel	Clonal analysis of hematopoietic progenitor cells in the zebrafish Clonal analysis of hematopoietic progenitor cells in the zebrafish (en)
skos:notation	RIV/68378050:_____/11:00365457!RIV12-AV0-68378050
http://linked.open...avai/predkladatel	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/08/0878), P(GAP305/10/0953), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Svoboda, Ondřej Bartůněk, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	190634
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/11:00365457
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	zebrafish; in vitro culture; hematopoiesis (en)
http://linked.open.../riv/klicoveSlovo	hematopoiesis in vitro culture zebrafish
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[BB037BC614DA]
http://linked.open...i/riv/nazevZdroje	Blood
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of cellular prion protein in erythroid differentiation: possible link to peripheral pathogenesis of prion diseases New regulators of megakaryocyte and erythroid lineage commitment
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	118
http://linked.open...iv/tvurceVysledku	Balla, K. M. Bartůněk, Petr Lau, R. P. Svoboda, Ondřej Stachura, D. L. Traver, D. Zon, L. I.
http://linked.open...ain/vavai/riv/wos	000293510000019
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0006-4971
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1182/blood-2011-01-331199
is http://linked.open...avai/riv/vysledek of	Clonal analysis of hematopoietic progenitor cells in the zebrafish Clonal analysis of hematopoietic progenitor cells in the zebrafish

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