About: Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity

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About: Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Homologous recombination (HR) provides an efficient mechanism for error-free repair of DNA double-strand breaks (DSBs). However, HR can be also harmful as inappropriate or untimely HR events can give rise to lethal recombination intermediates and chromosome rearrangements. A critical step of HR is the formation of a RAD51 filament on single-stranded (ss) DNA, which mediates the invasion of a homologous DNA molecule. In mammalian cells, several DNA helicases have been implicated in the regulation of this process. RECQ5, a member of the RecQ family of DNA helicases, interacts physically with the RAD51 recombinase and disrupts RAD51 presynaptic filaments in a reaction dependent on ATP hydrolysis. Here, we have precisely mapped the RAD51-interacting domain of RECQ5 and generated mutants that fail to interact with RAD51. We show that although these mutants retain normal ATPase activity, they are impaired in their ability to displace RAD51 from ssDNA. Homologous recombination (HR) provides an efficient mechanism for error-free repair of DNA double-strand breaks (DSBs). However, HR can be also harmful as inappropriate or untimely HR events can give rise to lethal recombination intermediates and chromosome rearrangements. A critical step of HR is the formation of a RAD51 filament on single-stranded (ss) DNA, which mediates the invasion of a homologous DNA molecule. In mammalian cells, several DNA helicases have been implicated in the regulation of this process. RECQ5, a member of the RecQ family of DNA helicases, interacts physically with the RAD51 recombinase and disrupts RAD51 presynaptic filaments in a reaction dependent on ATP hydrolysis. Here, we have precisely mapped the RAD51-interacting domain of RECQ5 and generated mutants that fail to interact with RAD51. We show that although these mutants retain normal ATPase activity, they are impaired in their ability to displace RAD51 from ssDNA. (en)
Title	Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity (en)
skos:prefLabel	Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity (en)
skos:notation	RIV/68378050:_____/10:00347098!RIV11-AV0-68378050
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	21
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Shevelev, Igor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	278913
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/10:00347098
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA helicase; double-strand breaks; homologous recombination (en)
http://linked.open.../riv/klicoveSlovo	homologous recombination DNA helicase double-strand breaks
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E2D22877AF1C]
http://linked.open...i/riv/nazevZdroje	Journal of Biological Chemistry
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	285
http://linked.open...iv/tvurceVysledku	Shevelev, Igor Janscak, P. Kanagaraj, R. Cheng, A. Paliwal, S. Raynard, S. Schwendener, S. Stark, J. M. Sung, P.
http://linked.open...ain/vavai/riv/wos	000277715900009
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0021-9258
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity Physical interaction of RECQ5 helicase with RAD51 facilitates its anti-recombinase activity

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