About: Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A PBS survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours of exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hrs. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed increased compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. PBS survival test with DHM opens a new approach to studying the structural integrity of neoplastic cells. Malignant cells appear to possess a special aptitude for survival. We attempted to prove this in vitro by acute nutritional and energy deprivation as a survival threat. A PBS survival test in cell culture allowed static observations. These were supplemented by classic and quantitative phase-contrast time-lapse microscopy. From one normal and four neoplastic cell populations, no cells survived 77 hours of exposure to PBS. Only G3S2 derived from a human breast carcinoma survived 60 hrs. Cells in sparse culture were more vulnerable than those in dense. Epithelial cells were more vigorous than mesenchymal. Cells of greater malignancy resisted longer. Evaluation in culture as detailed by digital holographic microscopy (DHM) revealed increased compactness of the intracellular mass motility from normal to metastasizing mesenchymal cells, thus reaching the level of epithelial G3S2 cells. PBS survival test with DHM opens a new approach to studying the structural integrity of neoplastic cells. (en)
Title	Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy (en)
skos:prefLabel	Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy (en)
skos:notation	RIV/68378050:_____/09:00334131!RIV10-AV0-68378050
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA202/08/0590), Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Veselý, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	337412
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/09:00334131
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	tumour cell; nutritional deprivation; energy deprivation; cell survival; cell death; digital holographic microscopy; dynamic phase difference (en)
http://linked.open.../riv/klicoveSlovo	tumour cell cell survival digital holographic microscopy dynamic phase difference cell death energy deprivation nutritional deprivation
http://linked.open...odStatuVydavatele	GR - Řecká republika
http://linked.open...ontrolniKodProRIV	[F9FF58792F56]
http://linked.open...i/riv/nazevZdroje	Anticancer Research
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Validation of digital holographic microscope in investigations of living cell dynamics & completion of new multifunctional instrument
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Veselý, Pavel Chmelík, R. Janečková, H.
http://linked.open...ain/vavai/riv/wos	000267389500061
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0250-7005
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy Proving tumour cells by acute nutritional/energy deprivation as a survival threat: a task for microscopy

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