About: BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

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About: BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Bloom syndrome (BS) is characterized by genomic instability and high predisposition to cancer. BLM, gene defective in BS, encodes a member of the RecQ family of 3´-5´ DNA helicases and is proposed to function in recombinational repair at DNA replication. We used single-molecule fluorescence resonance energy transfer microscopy to examine BLM behaviour on forked DNA substrates. BLM unwound individual DNA molecules repetitively, a short length unwound followed by rapid reannealing in successions. We show that monomeric BLM can %22measure%22 how many base pairs it has unwound, and after a critical length it reverses the unwinding through strand switching and translocating on the opposing strand. Repetitive unwinding persisted even in the presence of hRPA, and interaction between wild-type BLM and hRPA was necessary for unwinding reinitiation on hRPA-coated DNA. These activities may facilitate BLM processing of stalled replication forks and illegitimately formed recombination intermediates. Bloom syndrome (BS) is characterized by genomic instability and high predisposition to cancer. BLM, gene defective in BS, encodes a member of the RecQ family of 3´-5´ DNA helicases and is proposed to function in recombinational repair at DNA replication. We used single-molecule fluorescence resonance energy transfer microscopy to examine BLM behaviour on forked DNA substrates. BLM unwound individual DNA molecules repetitively, a short length unwound followed by rapid reannealing in successions. We show that monomeric BLM can %22measure%22 how many base pairs it has unwound, and after a critical length it reverses the unwinding through strand switching and translocating on the opposing strand. Repetitive unwinding persisted even in the presence of hRPA, and interaction between wild-type BLM and hRPA was necessary for unwinding reinitiation on hRPA-coated DNA. These activities may facilitate BLM processing of stalled replication forks and illegitimately formed recombination intermediates. (en)
Title	BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation (en)
skos:prefLabel	BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation (en)
skos:notation	RIV/68378050:_____/09:00333639!RIV10-AV0-68378050
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50520514)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Janščák, Pavel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav molekulární genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	305317
http://linked.open...ai/riv/idVysledku	RIV/68378050:_____/09:00333639
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bloom syndrome; FRET; helicase; hRPA; single molecule (en)
http://linked.open.../riv/klicoveSlovo	single molecule helicase FRET Bloom syndrome hRPA
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[72EE446B6FCC]
http://linked.open...i/riv/nazevZdroje	EMBO Journal
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Janščák, Pavel Kanagaraj, R. Yodh, J. G. Ha, T. Stevens, B. C.
http://linked.open...ain/vavai/riv/wos	000263556200012
http://linked.open...n/vavai/riv/zamer	Molekulárně genetické a buněčné základy klíčových biologických procesů: genová exprese, onkogeneze, replikace virů, imunita a vývoj organismů
issn	0261-4189
number of pages	12 (xsd:int)
is http://linked.open...avai/riv/vysledek of	BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation BLM helicase measures DNA unwound before switching strands and hRPA promotes unwinding reinitiation

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