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| rdf:type
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| Description
| - To identify HLA-E-specific Abs, four monoclonals of the previously described MEM series were screened by isoelectric focusing (IEF) blot and immunoprecipitation/IEF on >30 single-allele class I transfectants and HLA-homozygous B lymphoid cells coexpressing HLA-E and HLA-A, -B, -C, -F, or -G. All of the MEM Abs reacted with b2m-free and denatured (but not b2m-associated and folded) HLA-E H chains. Other HLA-E-restricted Abs were also reactive with free H chains. Several methods revealed the coexistence of HLA-E conformers with unusual features. MEM-reactive conformers were thermally unstable and poorly surface expressed, whereas b2m-associated conformers were either unstable and weakly reactive with the prototypic conformational Ab W6/32, or exceptionally stable and strongly reactive with Abs to b2m. Thus, HLA-E is a good b2m assembler, and TAP/tapasin-assisted ligand donation is only one, and possibly not even the major, pathway leading to its stabilization and surface expression.
- To identify HLA-E-specific Abs, four monoclonals of the previously described MEM series were screened by isoelectric focusing (IEF) blot and immunoprecipitation/IEF on >30 single-allele class I transfectants and HLA-homozygous B lymphoid cells coexpressing HLA-E and HLA-A, -B, -C, -F, or -G. All of the MEM Abs reacted with b2m-free and denatured (but not b2m-associated and folded) HLA-E H chains. Other HLA-E-restricted Abs were also reactive with free H chains. Several methods revealed the coexistence of HLA-E conformers with unusual features. MEM-reactive conformers were thermally unstable and poorly surface expressed, whereas b2m-associated conformers were either unstable and weakly reactive with the prototypic conformational Ab W6/32, or exceptionally stable and strongly reactive with Abs to b2m. Thus, HLA-E is a good b2m assembler, and TAP/tapasin-assisted ligand donation is only one, and possibly not even the major, pathway leading to its stabilization and surface expression. (en)
- Pomocí isoelektrické fokusace, Western blottingu a imunoprecipitace byla zkoumána specificita monoklonálních protilátek série MEM popsaných dříve jako reaktivních s HLA-E. Byla použita kolekce HLA-homozygotních B lymfoidních linií a transfektantů koexprimujících HLA-E a HLA-A, -B, -C, -F, nebo-G. Všechny protilátk reagovaly s denaturovanými volnými těžkými řetězci neasociovanými s b2m. Byla prokázána koexistence několika konformerů HLA-E s výrazně odlišnými vlastnostmi a buněčnou lokalizací. Konformery reaktivní s MEM-protilátkami byly nestabilní a málo exprimované na buněčném povrchu, zatímco konformery asociované s b2m byly v některých případech nestabilní, a v jiných vysoce stabilní a silně reaktivní s protilátkami proti b2m. HLA-E tedy asociuje dobře s b2m; mechanismy zodpovědné za asociaci s b2m nejsou zřejmě závislé pouze na dodání peptidů pomocí TAP/tapasinu. (cs)
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| Title
| - HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides
- HLA-E: silná asociace s beta2-mikroglobulinem a povrchová exprese v nepřítomnosti HLA peptidů I. třídy odvozených ze signální sekvence (cs)
- HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides (en)
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| skos:prefLabel
| - HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides
- HLA-E: silná asociace s beta2-mikroglobulinem a povrchová exprese v nepřítomnosti HLA peptidů I. třídy odvozených ze signální sekvence (cs)
- HLA-E: strong association with beta2-microglobulin and surface expression in the absence of HLA class I signal sequence-derived peptides (en)
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| skos:notation
| - RIV/68378050:_____/08:00318348!RIV09-AV0-68378050
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(EA 4.2PT01/064), Z(AV0Z50520514)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/08:00318348
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - HLA-E; MHC; monoclonal antibodies (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Hořejší, Václav
- Suchánek, M.
- Giacomini, P.
- Lo Monaco, E.
- Martayan, A.
- Melucci, E.
- Sibilio, L.
- Tremante, E.
|
| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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