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| Description
| - Byly studovány změny ve struktuře d(GGGA)5 oligonukleotidu v přítomnosti Li+, Na+ a K+ iontů pomocí elektronického a vibračního cirkulárního dichroismu, IR absorbce, ab initio výpočtu a dále charakterizovány elektroforeticky v nedenaturujícím prostředí. Oligonukleotid d(GGGA)5 tvořil v přítomnosti Li+ neplanární tetramer s úhly 102º a 171º mezi sousedními quaninovými bázemi. Tento tetramer mění svou geometrii při teplotách >50ºC, ale netvoří kvadruplexovou strukturu. V přítomnosti Na+ se struktura d(GGGA)5 optimalizovala do téměř planárních tetramerů s úhlem 177º mezi sousedními quaniny. Spektrální studie ukazují, že se hromadí do kvadruplexové helikální struktury a naopak rozkládají do jednoduchých tetramerů při teplotách >60ºC. Struktura d(GGGA)5 v přítomnosti jednomocných iontů je stabilizovaná proti teplotní denaturaci v pořadí Li+ < Na+ < K+. (cs)
- Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, ab initio calculations and further characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5 in the presence of Li+ forms a nonplanar single tetramer, with angles of 102º and 171º between neighboring guanine bases. This tetramer changes its geometry at temperatures >50ºC, but does not form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177º between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structure decaying to a single tetramer at temperatures >60ºC. The d(GGGA)5 structure in the presence of monovalent ions is stabilized against thermal denaturation in the order Li+ < Na+ < K+.
- Variations in the structure of d(GGGA)5 oligonucleotide in the presence of Li+, Na+, and K+ ions and its temperature stability were studied using electronic and vibrational circular dichroism, IR absorption, ab initio calculations and further characterized by nondenaturing gel electrophoresis. Oligonucleotide d(GGGA)5 in the presence of Li+ forms a nonplanar single tetramer, with angles of 102º and 171º between neighboring guanine bases. This tetramer changes its geometry at temperatures >50ºC, but does not form a quadruplex structure. In the presence of Na+, the d(GGGA)5 structure was optimized to almost planar tetramers with an angle of 177º between neighboring guanines. The spectral results suggest that it stacks into a quadruplex helical structure decaying to a single tetramer at temperatures >60ºC. The d(GGGA)5 structure in the presence of monovalent ions is stabilized against thermal denaturation in the order Li+ < Na+ < K+. (en)
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| Title
| - Formation and temperature stability of G-quadruplex structures studied by electronic and vibrational dichroism spectroscopy combined with ab initio calculations
- Tvorba a teplotní stabilita G-kvadruplexních struktur studovaných spektroskopií elektronického a vibračního cirkulárního dichroismu v kombinaci s ab initio výpočtem (cs)
- Formation and temperature stability of G-quadruplex structures studied by electronic and vibrational dichroism spectroscopy combined with ab initio calculations (en)
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| skos:prefLabel
| - Formation and temperature stability of G-quadruplex structures studied by electronic and vibrational dichroism spectroscopy combined with ab initio calculations
- Tvorba a teplotní stabilita G-kvadruplexních struktur studovaných spektroskopií elektronického a vibračního cirkulárního dichroismu v kombinaci s ab initio výpočtem (cs)
- Formation and temperature stability of G-quadruplex structures studied by electronic and vibrational dichroism spectroscopy combined with ab initio calculations (en)
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| skos:notation
| - RIV/68378050:_____/08:00097980!RIV08-AV0-68378050
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/08:00097980
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - vibrational circular dichroism; quadruplex; G-rich nucleotide (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Král, V.
- Králová, Jarmila
- Urbanová, M.
- Nový, J.
- Böhm, S.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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