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| rdf:type
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| Description
| - V souvislosti s možností xenotransplantace prasečích orgánů lidem je zdůrazňováno nebezpečí zoonotické infekce prasečími endogenními retroviry (PERV). Podskupina PERV-A může infikovat lidské buňky pomocí dvou homologních transmembránových proteinů, huPAR-1 a huPAR-2. V této práci jsme charakterizovali lidské PAR a jejich homology u myši a krysy (muPAR a ratPAR). Krysí i myší buňky jsou rezistentní vůči PERV-A. Transfekce huPAR a ratPAR receptorů navodila citlivost k PERV-A u různých resistentních buněk, transfekce muPAR resistenci neovlivnila. Zjistili jsme, že rezistence krysích buněk je daná nízkou endogenní expresí ratPAR. Rezistence myších buněk je způsobena přítomností Pro v pozici 109 v druhé extracelulární smyčce muPAR, který zabraňuje vazbě PERV-A Env na receptor. Z této studie vyplývá, že oba druhy hlodavců jsou rezistentní vůči PERV-A, rezistenci však získaly nezávisle, různými způsoby. (cs)
- The risk of zoonotic infection by porcine endogenous retroviruses (PERV) has been highlighted in the context of pig-to-human xenotransplantation. A human-tropic PERV subgroup, PERV-A, can enter human cells through either of two homologous transmembrane proteins, huPAR-1 and huPAR-2. Here, we characterised human PARs and their homologues in the PERV-A resistant rodent species, mouse and rat (muPAR and ratPAR). Upon exogenous expression in PERV-A resistant cells, human and rat PARs, but not muPAR, conferred PERV-A sensitivity. Exogenously expressed ratPAR allows PERV-A infection with equivalent efficiency to that of huPAR-1. Endogenous ratPAR expression in rat cell lines appeared to be too low for PERV-A infection. In contrast, in mouse the PERV-A resistance is caused by the presence of Pro at position 109 in the second extracelular loop of muPAR, which affects the PERV-A Env binding to PAR molecules. Both rodent species are PERV-A resistant, however, on different basis.
- The risk of zoonotic infection by porcine endogenous retroviruses (PERV) has been highlighted in the context of pig-to-human xenotransplantation. A human-tropic PERV subgroup, PERV-A, can enter human cells through either of two homologous transmembrane proteins, huPAR-1 and huPAR-2. Here, we characterised human PARs and their homologues in the PERV-A resistant rodent species, mouse and rat (muPAR and ratPAR). Upon exogenous expression in PERV-A resistant cells, human and rat PARs, but not muPAR, conferred PERV-A sensitivity. Exogenously expressed ratPAR allows PERV-A infection with equivalent efficiency to that of huPAR-1. Endogenous ratPAR expression in rat cell lines appeared to be too low for PERV-A infection. In contrast, in mouse the PERV-A resistance is caused by the presence of Pro at position 109 in the second extracelular loop of muPAR, which affects the PERV-A Env binding to PAR molecules. Both rodent species are PERV-A resistant, however, on different basis. (en)
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| Title
| - Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species
- Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species (en)
- Různé formy rezistence hlodavců vůči vstupu prasečího endogenního retroviru podskupiny A do buňky (cs)
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| skos:prefLabel
| - Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species
- Differential resistance to cell entry by porcine endogenous retrovirus subgroup A in rodent species (en)
- Různé formy rezistence hlodavců vůči vstupu prasečího endogenního retroviru podskupiny A do buňky (cs)
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| skos:notation
| - RIV/68378050:_____/07:00306859!RIV09-AV0-68378050
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/07:00306859
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - PERV; xenotransplantation; receptor (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Matoušková, Magda
- Takeuchi, Y.
- Mattiuzzo, G.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| is http://linked.open...avai/riv/vysledek
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