| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - We investigated if malignant cells in situ additionally characterized by increased numbers of proto-oncogenes can be traced by the FISH method in the ex vivo-derived cultures. In 6 tumors (5 primary tumors, 1 cutaneous metastasis), increased numbers of FISH signals were found in 55-99% of cells. In cell populations maintained for up to 2-6 passages in vitro the incidence of cells with increased FISH signals was found to be low (2-16%). Moreover, the cells with multiplied signals that survived more than one passage in vitro were evidently unable to divide further. However, in all 6 tumors at least a small fraction of cells displaying only two signals of CCND1 or HER2/neu genes was identified directly in invasive tumor structures in the vicinity of cells with multiple signals. Our findings suggest that these invasive tumor cells displaying only two proto-oncogene signals were most probably involved in the initiation and propagation of ex vivo tumor-derived primary cell cultures.
- We investigated if malignant cells in situ additionally characterized by increased numbers of proto-oncogenes can be traced by the FISH method in the ex vivo-derived cultures. In 6 tumors (5 primary tumors, 1 cutaneous metastasis), increased numbers of FISH signals were found in 55-99% of cells. In cell populations maintained for up to 2-6 passages in vitro the incidence of cells with increased FISH signals was found to be low (2-16%). Moreover, the cells with multiplied signals that survived more than one passage in vitro were evidently unable to divide further. However, in all 6 tumors at least a small fraction of cells displaying only two signals of CCND1 or HER2/neu genes was identified directly in invasive tumor structures in the vicinity of cells with multiple signals. Our findings suggest that these invasive tumor cells displaying only two proto-oncogene signals were most probably involved in the initiation and propagation of ex vivo tumor-derived primary cell cultures. (en)
- Snažili jsme se zjistit, zda maligní buňky in situ charakterizované ještě zvýšeným počtem protoonkogenů mohou být v ex vivo odvozených kulturách zachyceny metodou FISH. V 6 nádorech (5 primárních nádorů, 1 kožní metastáza) byly zvýšené signály FISH nalezeny v 55-99% buněk. V buněčných populacích udržených až 2-6 pasáží in vitro byl výskyt nalezených zvýšených signálů FISH nízký (2-16%). Navíc buňky se znásobenými signály, které přežily více než jednu pasáž in vitro, se už evidentně nemohly množit. Ve všech 6 nádorech však byla přímo v invazivních strukturách poblíž buněk se znásobenými signály identifikována alespoň malá frakce buněk vykazujících pouze dva signály genů CCND1 či HER2/neu. Naše výsledky naznačují, že invazivní nádorové buňky vykazující pouze dva protoonkogenní signály se pravděpodobně podílely na iniciaci a propagaci primárních buněčných kultur odvozených ex vivo. (cs)
|
| Title
| - Origin of cells cultured in vitro from human breast carcinomas traced by cyclin D1 and HER2/neu FISH signal numbers
- Origin of cells cultured in vitro from human breast carcinomas traced by cyclin D1 and HER2/neu FISH signal numbers (en)
- Původ buněk kultivovaných in vitro z buněk lidského karcinomu prsu zjišťovaný počtem signálů FISH pro cyklin D1 a HER2/neu (cs)
|
| skos:prefLabel
| - Origin of cells cultured in vitro from human breast carcinomas traced by cyclin D1 and HER2/neu FISH signal numbers
- Origin of cells cultured in vitro from human breast carcinomas traced by cyclin D1 and HER2/neu FISH signal numbers (en)
- Původ buněk kultivovaných in vitro z buněk lidského karcinomu prsu zjišťovaný počtem signálů FISH pro cyklin D1 a HER2/neu (cs)
|
| skos:notation
| - RIV/68378050:_____/05:00001378!RIV06-AV0-68378050
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(NR8145), Z(AV0Z50520514)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/68378050:_____/05:00001378
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - breast carcinomas; primary cultures of carcinoma cells; cyclin D1 and HER2/neu by FISH (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Veselý, Pavel
- Matoušková, Eva
- Brožová, Markéta
- Chaloupková, Alena
- Kudláčková, Iva
- Netíková, I.
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| is http://linked.open...avai/riv/vysledek
of | |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)