About: Human Embryonic Stem Cells Suffer from Centrosomal Amplification

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Propagation of human embryonic stem cells (hESCs) in culture tends to alter karyotype, potentially limiting the prospective use of these cells in patients. The chromosomal instability of some malignancies is considered to be driven, at least in part, by centrosomal overamplification, perturbing balanced chromosome segregation. Here, we report, for the first time, that very high percentage of cultured hESCs has supernumerary centrosomes during mitosis. Supernumerary centrosomes were strictly associated with an undifferentiated hESC state and progressively disappeared on prolonged propagation in culture. Improved attachment to culture substratum and inhibition of CDK2 and Aurora A (key regulators of centrosomal metabolism) diminished the frequency of multicentrosomal mitoses. Collectively, our data indicate that supernumerary centrosomes are a significant risk factor for chromosome instability in cultured hESCs and should be evaluated when new culture conditions are being implemented. Propagation of human embryonic stem cells (hESCs) in culture tends to alter karyotype, potentially limiting the prospective use of these cells in patients. The chromosomal instability of some malignancies is considered to be driven, at least in part, by centrosomal overamplification, perturbing balanced chromosome segregation. Here, we report, for the first time, that very high percentage of cultured hESCs has supernumerary centrosomes during mitosis. Supernumerary centrosomes were strictly associated with an undifferentiated hESC state and progressively disappeared on prolonged propagation in culture. Improved attachment to culture substratum and inhibition of CDK2 and Aurora A (key regulators of centrosomal metabolism) diminished the frequency of multicentrosomal mitoses. Collectively, our data indicate that supernumerary centrosomes are a significant risk factor for chromosome instability in cultured hESCs and should be evaluated when new culture conditions are being implemented. (en)
Title	Human Embryonic Stem Cells Suffer from Centrosomal Amplification Human Embryonic Stem Cells Suffer from Centrosomal Amplification (en)
skos:prefLabel	Human Embryonic Stem Cells Suffer from Centrosomal Amplification Human Embryonic Stem Cells Suffer from Centrosomal Amplification (en)
skos:notation	RIV/68378041:_____/11:00367964!RIV12-AV0-68378041
http://linked.open...avai/predkladatel	Ústav experimentální medicíny Akademie věd České republiky, v. v. i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(2B06052), P(GA204/09/2044), Z(AV0Z50390703), Z(MSM0021622430)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Hampl, Aleš Bárta, Tomáš Doležalová, Dáša Vinarský, Vladimír Dvořák, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální medicíny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	202961
http://linked.open...ai/riv/idVysledku	RIV/68378041:_____/11:00367964
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	human embryonic stem cells; centrosome; chromosome (en)
http://linked.open.../riv/klicoveSlovo	chromosome centrosome human embryonic stem cells
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[92D6BE0FDA3A]
http://linked.open...i/riv/nazevZdroje	Stem Cells
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Determination of markers, screening and early diagnostics of cancer diseases using highly automated processing of multidimensional biomedical images Molecular sources of centrosomal abnormalities in human embryonic stem cells
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	29
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Hampl, Aleš Bárta, Tomáš Doležalová, Dáša Holubcová, Z. Matula, P. Sedláčková, M. Vinarský, Vladimír
http://linked.open...ain/vavai/riv/wos	000286659300007
http://linked.open...n/vavai/riv/zamer	New biotechnologies, nanomaterials and stem cells for use in regenerative medicine Functional and molecular characteristics of cancer and normal stem cells - identification of targets for novel therapeutics and therapeutic strategies
issn	1066-5099
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/stem.549
is http://linked.open...avai/riv/vysledek of	Human Embryonic Stem Cells Suffer from Centrosomal Amplification Human Embryonic Stem Cells Suffer from Centrosomal Amplification

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