About: Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums

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About: Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	V muzeích se nacházejí rozsáhlé sbírky savčích kůží a my jsme určili možnosti jejich využití při evolučních a ochranářských studiích využívajících DNA. Na zpracování vzorků kůží hranostajů (Mustela erminea) z 18 muzeí v 11 zemích jsme použili metody odvozené od přístupu k prastaré DNA. Úspěšné polymerázové řetězové reakce (PCR) byly zaznamenány v 56%, 46% a 40% vzorků pro PCR produkty z mitochondriální DNA dlouhé 0,65; 0,7 a 0,78 kb. Některé z nejlépe zachovalých vzorků kůží byly skladovány v těsnících kontejnerech a v chladném prostředí. Vzorky savčích kůží mohou být dobrými zdroji DNA za předpokladu vhodné prezervace. (cs) There are large museum collections of mammalian skins and we wished to determine their usefulness for DNA-based evolutionary and conservation studies. Methods derived from the ancient DNA approach were used to process samples from skins of the stoat (Mustela erminea) from 18 museums in 11 countries. Successful polymerase chain reaction (PCR) amplification was achieved in 56%, 46% and 40% of all samples for 0.65-, 0.70- and 0.78-kb PCR products of mitochondrial DNA, respectively. Some of the best-preserved skin samples were those in tight-fitting containers in a dry and cold environment. With care in their preservation, mammalian skin collections may be a good source of DNA. There are large museum collections of mammalian skins and we wished to determine their usefulness for DNA-based evolutionary and conservation studies. Methods derived from the ancient DNA approach were used to process samples from skins of the stoat (Mustela erminea) from 18 museums in 11 countries. Successful polymerase chain reaction (PCR) amplification was achieved in 56%, 46% and 40% of all samples for 0.65-, 0.70- and 0.78-kb PCR products of mitochondrial DNA, respectively. Some of the best-preserved skin samples were those in tight-fitting containers in a dry and cold environment. With care in their preservation, mammalian skin collections may be a good source of DNA. (en)
Title	Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums Úspěšnost amplifikace DNA z muzejních kožních preparátů: studie hranostajů z 18 muzeí (cs) Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums (en)
skos:prefLabel	Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums Úspěšnost amplifikace DNA z muzejních kožních preparátů: studie hranostajů z 18 muzeí (cs) Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums (en)
skos:notation	RIV/68081766:_____/06:00043746!RIV07-AV0-68081766
http://linked.open.../vavai/riv/strany	1014;1017
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z60930519)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Martínková, Natália
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav biologie obratlovců AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	464730
http://linked.open...ai/riv/idVysledku	RIV/68081766:_____/06:00043746
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ancient DNA; mitochondrial DNA,; Mustela erminea; museum collections; polymerase chain reaction (en)
http://linked.open.../riv/klicoveSlovo	ancient DNA polymerase chain reaction mitochondrial DNA museum collections Mustela erminea
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FA9C7B799F69]
http://linked.open...i/riv/nazevZdroje	Molecular Ecology Notes
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Martínková, Natália Searle, J. B.
http://linked.open...n/vavai/riv/zamer	Biodiverzita a ekologie obratlovců: Strategie ochrany a využívání přírodních populací
issn	1471-8278
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums Amplification success rate of DNA from museum skin collections: a case study of stoats from 18 museums

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