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  • The size-based separations of single-stranded DNA fragments under denaturing conditions are more selective than the separations of their double-stranded analogs. With respect to the denaturing ability, NaOH is probably the strongest denaturing agent. Thus, even the DNA fragments with GC rich regions, which are resistant to the denaturing in urea, dissociate completely at pH over 12.
  • The size-based separations of single-stranded DNA fragments under denaturing conditions are more selective than the separations of their double-stranded analogs. With respect to the denaturing ability, NaOH is probably the strongest denaturing agent. Thus, even the DNA fragments with GC rich regions, which are resistant to the denaturing in urea, dissociate completely at pH over 12. (en)
Title
  • Alkaline Capillary Electrophoresis.
  • Alkaline Capillary Electrophoresis. (en)
skos:prefLabel
  • Alkaline Capillary Electrophoresis.
  • Alkaline Capillary Electrophoresis. (en)
skos:notation
  • RIV/68081715:_____/02:25033052!RIV/2004/GA0/A25004/N
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  • 33
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  • P(GA203/00/0772), P(GA303/00/0928), Z(AV0Z4031919)
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  • 637796
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  • RIV/68081715:_____/02:25033052
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  • single strand conformation polymorphism; single cell analysis (en)
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  • [0D880375D267]
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  • Helsinki [FI]
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  • Helsinki
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  • ITP 2002. The 13th International Symposium on Capillary Electroseparation Techniques. Program and Book of Abstracts.
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  • Klepárník, Karel
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  • Neuveden
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