About: The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work we have studied epigenetic silencing induced by an invertedly repeated transgenic locus. We found that its posttranscriptionally silenced epilalle efficiently trans-silenced and trans-methylated any homologous locus while the transcriptionally variant was inactive. RNA rather than DNA molecules are therefore needed for trans interactions of homologous loci in cell nucleus. In this work we have studied epigenetic silencing induced by an invertedly repeated transgenic locus. We found that its posttranscriptionally silenced epilalle efficiently trans-silenced and trans-methylated any homologous locus while the transcriptionally variant was inactive. RNA rather than DNA molecules are therefore needed for trans interactions of homologous loci in cell nucleus. (en) V této práci jsme studovali epigenetické umlčování indukované transgenním lokusem, který byl uspořádán jako obrácená repetice. Zjistili jsme, že jeho posttranskripčně umlčená epialela efektivně umlčuje a metyluje homologní sekvence DNA, zatímco jeho transkripčně umlčená varianta tento proces neumožňuje. Výsledek podporuje hypotézu, že pro navození interakcí homologních lokusů v jádře jsou zapotřebí molekuly RNA. (cs)
Title	The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco Umlčovací schopnost transgenů uspořádaných jako obrácená repetice závisí na jejich epigenetickém stavu (cs) The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco (en)
skos:prefLabel	The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco Umlčovací schopnost transgenů uspořádaných jako obrácená repetice závisí na jejich epigenetickém stavu (cs) The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco (en)
skos:notation	RIV/68081707:_____/06:00038955!RIV07-AV0-68081707
http://linked.open.../vavai/riv/strany	2280;2293
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/04/0775), P(GD204/05/H505), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Kovařík, Aleš Fojtová, Miloslava Křížová, Kateřina Lunerová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	504296
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/06:00038955
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	transgenes; epigenetic silencing; DNA methylation (en)
http://linked.open.../riv/klicoveSlovo	transgenes epigenetic silencing DNA methylation
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[36512195AC0B]
http://linked.open...i/riv/nazevZdroje	Nucleic Acids Research
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Epigenetic regulation of gene expression in transgenic and endogenous loci of higher plants Developmental and evolutionary genetics of plants
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	34
http://linked.open...iv/tvurceVysledku	Křížová, Kateřina Kovařík, Aleš Fojtová, Miloslava Depicker, A. Bleys, A. Van Houdt, H. Bedřichová, Jana
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems
issn	0305-1048
number of pages	14 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco The trans-silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco

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