About: Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells

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About: Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this paper, we propose a new voltammetric method for the detection of the DNA base damage based on enzymatic conversion of the damaged DNA bases to single-strand breaks (ssb), single-stranded (ss) DNA regions, or both. Supercoiled DNA exposed to UV light was specifically cleaved by T4 endonuclease V, an enzyme recognizing pyrimidine dimers, the major products of photochemical DNA damage. Apurinic sites (formed in dimethyl sulfate - modified DNA) were determined after treating the DNA with E. coli exonuclease III, an enzyme introducing ssb at the abasic sites and degrading one of the DNA strands. The ssb or ssDNA regions, or both, were detected by adsorptive transfer stripping alternating current voltammetry at the mercury electrode. This technique offers much better sensitivity and selectivity of DNA base damage detection than any other electrochemical method and it can detect also DNA base damage induced in living bacterial cells. In this paper, we propose a new voltammetric method for the detection of the DNA base damage based on enzymatic conversion of the damaged DNA bases to single-strand breaks (ssb), single-stranded (ss) DNA regions, or both. Supercoiled DNA exposed to UV light was specifically cleaved by T4 endonuclease V, an enzyme recognizing pyrimidine dimers, the major products of photochemical DNA damage. Apurinic sites (formed in dimethyl sulfate - modified DNA) were determined after treating the DNA with E. coli exonuclease III, an enzyme introducing ssb at the abasic sites and degrading one of the DNA strands. The ssb or ssDNA regions, or both, were detected by adsorptive transfer stripping alternating current voltammetry at the mercury electrode. This technique offers much better sensitivity and selectivity of DNA base damage detection than any other electrochemical method and it can detect also DNA base damage induced in living bacterial cells. (en)
Title	Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells (en)
skos:prefLabel	Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells Use of DNA repair enzymes in electrochemical detection of damage to DNA bases in vitro and in cells (en)
skos:notation	RIV/68081707:_____/05:00373435!RIV12-AV0-68081707
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1H-PK/42), P(GA203/04/1325), Z(AV0Z50040507), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Cahová, Kateřina Paleček, Emil Mozga, Tomáš Fojta, Miroslav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	548060
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/05:00373435
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA damage; voltammetry; repair enzymes (en)
http://linked.open.../riv/klicoveSlovo	repair enzymes DNA damage voltammetry
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[14EA5BEF046B]
http://linked.open...i/riv/nazevZdroje	Analytical Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Research and development of a new type of electrochemical biosensor for the detection of nucleotide sequences in DNA and of genotoxic agents in the environment. New approaches in development of electrochemical sensors for DNA damage
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	77
http://linked.open...iv/tvurceVysledku	Fojta, Miroslav Paleček, Emil Mozga, Tomáš Cahová-Kuchaříková, Kateřina
http://linked.open...ain/vavai/riv/wos	000228877500044
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0003-2700
number of pages	8 (xsd:int)

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