About: Sequestering of p53 into DNA-protein filaments revealed by electron microscopy

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed. Using electron microscopy, we analyzed the interaction of bacterially expressed full-length p53, p53(1-393), and its C-terminal fragment, p53(320-393), with long (~3000 bp) dsDNA in linear and supercoiled forms containing or lacking the p53 recognition sequence (p53CON). The main structural feature of the complexes formed by either protein was a DNA-protein filament, in which two DNA duplexes are linked (synapsed) via bound protein tetramers. The efficiency of the synapse, reflected in its length and the fraction of molecules exhibiting DNA-protein filaments, was significantly modulated by the molecular form of the protein and the topological state of the DNA. Possible implications for the sequestering of p53 in DNA-protein filaments are discussed. (en) Pomocí elektronové mikroskopie byly analyzovány interakce proteinů p53 exprimovaných v bakteriích, celého proteinu p53, p53(1-393) a C-terminálního fragmentu p53(320-393), s dlouhou dsDNA (přibližně 3000 bp) v lineární a superhelikální formě obsahující či neobsahující proteinem p53 rozpoznávanou sekvenci (p53CON). Hlavním strukturním znakem vytvořených komplexů byl DNA -protein filament, ve kterém jsou duplexy DNA spojeny díky navázanému tetrametru proteinu. V práci byly diskutovány možné důsledky zachycení p53 prostřednictvím tvorby těchto DNA-proteinových filament. (cs)
Title	Sequestering of p53 into DNA-protein filaments revealed by electron microscopy Zachycení p53 prostřednictvím filament DNA-protein zobrazené elektronovou (cs) Sequestering of p53 into DNA-protein filaments revealed by electron microscopy (en)
skos:prefLabel	Sequestering of p53 into DNA-protein filaments revealed by electron microscopy Zachycení p53 prostřednictvím filament DNA-protein zobrazené elektronovou (cs) Sequestering of p53 into DNA-protein filaments revealed by electron microscopy (en)
skos:notation	RIV/68081707:_____/05:00001223!RIV06-AV0-68081707
http://linked.open.../vavai/riv/strany	261;271
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/02/0831), P(GA301/99/D078), P(IAA4004110), P(IAA500040513), Z(AV0Z50040507)
http://linked.open...iv/cisloPeriodika	2-3
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Paleček, Emil Paleček, Jan Brázdová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	542394
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/05:00001223
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	p53; electron microscopy; DNA-protein complex (en)
http://linked.open.../riv/klicoveSlovo	electron microscopy p53 DNA-protein complex
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[DAD38E2973DF]
http://linked.open...i/riv/nazevZdroje	Biophysical Chemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Interactions of p53 protein and its homologues with DNA and their role in malignant transformation Role of the p53 domains and the oligomerization state of the protein in its molecular interactions Binding of tumor suppressor protein p53 to DNA. Effect of DNA supercoling and protein postsynthetic modification Wild type and mutant p53 tumor suppressor protein. Intermolecular interactions, changes in conformation and new micro-methods of their analysis
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	14
http://linked.open...iv/tvurceVysledku	Brázdová, Marie Paleček, Emil Paleček, Jan Jovin, T. M. Cherny, Dmitry I.
http://linked.open...n/vavai/riv/zamer	Biophysics of dynamic structures and functions of biological systems
issn	0301-4622
number of pages	11 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Sequestering of p53 into DNA-protein filaments revealed by electron microscopy

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