About: Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393).

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About: Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Tumor suppressor protein p53 is often expressed as a fusion protein with glutathione-S-transferase (GST). The sensitive determination of GST in p53 samples is thus necessary. We propose a method for the determination of traces of GST in the p53 C-terminus based on the constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE). GST produces a catalytic signal in cobalt-containing solutions due to cysteine residues. A large excess of the C-terminus does interfere with the determination because of the lack of cysteines in the molecule. This method is simple and very sensitive and is capable of detecting <1% GST in the p53 sample. Tumor suppressor protein p53 is often expressed as a fusion protein with glutathione-S-transferase (GST). The sensitive determination of GST in p53 samples is thus necessary. We propose a method for the determination of traces of GST in the p53 C-terminus based on the constant current chronopotentiometric stripping analysis (CPSA) with hanging mercury drop electrode (HMDE). GST produces a catalytic signal in cobalt-containing solutions due to cysteine residues. A large excess of the C-terminus does interfere with the determination because of the lack of cysteines in the molecule. This method is simple and very sensitive and is capable of detecting <1% GST in the p53 sample. (en)
Title	Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). (en)
skos:prefLabel	Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). Determination of glutathione-S-transferase traces in preparations of p53 C-terminal domain (aa320-393). (en)
skos:notation	RIV/68081707:_____/02:17023049!RIV/2003/GA0/A17003/N
http://linked.open.../vavai/riv/strany	115;118
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/00/D049), P(GV204/97/K084), P(IAA4004110), P(NC5343), Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	1/2
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Paleček, Emil Havran, Luděk Kizek, René Brázdová, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Biofyzikální ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	642721
http://linked.open...ai/riv/idVysledku	RIV/68081707:_____/02:17023049
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	p53; glutathione-S-transferase determination; constant current chronopotentiometry (en)
http://linked.open.../riv/klicoveSlovo	p53 constant current chronopotentiometry glutathione-S-transferase determination
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[83292A39FEAB]
http://linked.open...i/riv/nazevZdroje	Bioelectrochemistry
http://linked.open...in/vavai/riv/obor	BO
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...vavai/riv/projekt	Influence of chemical modification of DNA and synthetic oligonucleotides on their electrochemical behaviour Electrodes modified with nucleic acids and proteins.New tools in biochemical and biomedical research. Interaction of tumor supressor protein p53 with damaged DNA and with DNA lesions induced by anti-cancer drugs Binding of tumor suppressor protein p53 to DNA. Effect of DNA supercoling and protein postsynthetic modification
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Brázdová, Marie Havran, Luděk Kizek, René Paleček, Emil
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	1567-5394
number of pages	4 (xsd:int)

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